miRNA: 3'- gGCCGUAGCucuAGuAGGCGGACGGC- -5'
miRNA: 3'- -GGCCGu------------AGCu-CUAGUaGGCGGACG-GC- -5'
miRNA: 3'- -GGCCGUAGCUCUAGUAGGCGGACGGC- -5'
miRNA: 3'- -GGCCguA-GCUCUAgUAGgCGGAC-GGC- -5'
miRNA: 3'- -GGcCGUaGCUCUAGUAGGCGgACGGC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22735 | 3' | -57 | NC_005091.1 | + | 53555 | 0.69 | 0.49066 |
|
Target: 5'- gCGGUGUCG---UCuUCCGCUUGCCGc -3' miRNA: 3'- gGCCGUAGCucuAGuAGGCGGACGGC- -5' |
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| 22735 | 3' | -57 | NC_005091.1 | + | 55693 | 0.68 | 0.527347 |
|
Target: 5'- cCCGGCuugccgcaucacuUCGccGAUCG-CCGCCUGCaCGa -3' miRNA: 3'- -GGCCGu------------AGCu-CUAGUaGGCGGACG-GC- -5' |
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| 22735 | 3' | -57 | NC_005091.1 | + | 56203 | 1.11 | 0.000611 |
|
Target: 5'- uCCGGCAUCGAGAUCAUCCGCCUGCCGg -3' miRNA: 3'- -GGCCGUAGCUCUAGUAGGCGGACGGC- -5' |
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| 22735 | 3' | -57 | NC_005091.1 | + | 57332 | 0.7 | 0.451348 |
|
Target: 5'- gCCGGguUgCGGGAUgAUCgGCUUGuCCGg -3' miRNA: 3'- -GGCCguA-GCUCUAgUAGgCGGAC-GGC- -5' |
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| 22735 | 3' | -57 | NC_005091.1 | + | 57720 | 0.8 | 0.107471 |
|
Target: 5'- gCCuGCA-CGAGAUCGUCCGCgUGCUGa -3' miRNA: 3'- -GGcCGUaGCUCUAGUAGGCGgACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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