Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22735 | 3' | -57 | NC_005091.1 | + | 33645 | 0.68 | 0.552365 |
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Target: 5'- gCGGCAUCGGGAcuUCA-CCaGCCgcgauaGCCu -3' miRNA: 3'- gGCCGUAGCUCU--AGUaGG-CGGa-----CGGc -5' |
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| 22735 | 3' | -57 | NC_005091.1 | + | 11202 | 0.68 | 0.531489 |
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Target: 5'- gCGGCAUCGGGA-CuUCCGCUUucgggggagGCCc -3' miRNA: 3'- gGCCGUAGCUCUaGuAGGCGGA---------CGGc -5' |
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| 22735 | 3' | -57 | NC_005091.1 | + | 52063 | 0.68 | 0.521157 |
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Target: 5'- gCCGcGCGcuUCGAGAUauUCCGCCgcauuggcgcgGCCGu -3' miRNA: 3'- -GGC-CGU--AGCUCUAguAGGCGGa----------CGGC- -5' |
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| 22735 | 3' | -57 | NC_005091.1 | + | 46042 | 0.68 | 0.518073 |
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Target: 5'- gCGGCGggGAGAUCgauuacgacgacccGUUCGCC-GCCGa -3' miRNA: 3'- gGCCGUagCUCUAG--------------UAGGCGGaCGGC- -5' |
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| 22735 | 3' | -57 | NC_005091.1 | + | 56203 | 1.11 | 0.000611 |
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Target: 5'- uCCGGCAUCGAGAUCAUCCGCCUGCCGg -3' miRNA: 3'- -GGCCGUAGCUCUAGUAGGCGGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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