Results 21 - 40 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22735 | 3' | -57 | NC_005091.1 | + | 11202 | 0.68 | 0.531489 |
Target: 5'- gCGGCAUCGGGA-CuUCCGCUUucgggggagGCCc -3' miRNA: 3'- gGCCGUAGCUCUaGuAGGCGGA---------CGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 55693 | 0.68 | 0.527347 |
Target: 5'- cCCGGCuugccgcaucacuUCGccGAUCG-CCGCCUGCaCGa -3' miRNA: 3'- -GGCCGu------------AGCu-CUAGUaGGCGGACG-GC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 52063 | 0.68 | 0.521157 |
Target: 5'- gCCGcGCGcuUCGAGAUauUCCGCCgcauuggcgcgGCCGu -3' miRNA: 3'- -GGC-CGU--AGCUCUAguAGGCGGa----------CGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 46042 | 0.68 | 0.518073 |
Target: 5'- gCGGCGggGAGAUCgauuacgacgacccGUUCGCC-GCCGa -3' miRNA: 3'- gGCCGUagCUCUAG--------------UAGGCGGaCGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 25655 | 0.68 | 0.510905 |
Target: 5'- gCUGGCcgCGcAGuUCuUCCGCCUGCa- -3' miRNA: 3'- -GGCCGuaGC-UCuAGuAGGCGGACGgc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 39496 | 0.68 | 0.510905 |
Target: 5'- uUGGCAUgGGGAagacggUCAUCUGCCUcacGCUGc -3' miRNA: 3'- gGCCGUAgCUCU------AGUAGGCGGA---CGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 53555 | 0.69 | 0.49066 |
Target: 5'- gCGGUGUCG---UCuUCCGCUUGCCGc -3' miRNA: 3'- gGCCGUAGCucuAGuAGGCGGACGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 1385 | 0.69 | 0.470795 |
Target: 5'- cCCGGCGU---GAUCGaCUGCUUGCCGc -3' miRNA: 3'- -GGCCGUAgcuCUAGUaGGCGGACGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 5628 | 0.7 | 0.451348 |
Target: 5'- aCGGUAUUGAcGAgCGUCaggGCCUGCCu -3' miRNA: 3'- gGCCGUAGCU-CUaGUAGg--CGGACGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 8366 | 0.7 | 0.451348 |
Target: 5'- aCCGGCAUau---UCAUCUGCCggGCCa -3' miRNA: 3'- -GGCCGUAgcucuAGUAGGCGGa-CGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 57332 | 0.7 | 0.451348 |
Target: 5'- gCCGGguUgCGGGAUgAUCgGCUUGuCCGg -3' miRNA: 3'- -GGCCguA-GCUCUAgUAGgCGGAC-GGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 18174 | 0.7 | 0.432349 |
Target: 5'- cUCGGCAUCGAGAacaUgAUCCuGCgC-GCCGg -3' miRNA: 3'- -GGCCGUAGCUCU---AgUAGG-CG-GaCGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 44227 | 0.7 | 0.432349 |
Target: 5'- aCCGGCAUaCGccccGAUCAUCUGCUcgGCaCGg -3' miRNA: 3'- -GGCCGUA-GCu---CUAGUAGGCGGa-CG-GC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 5074 | 0.7 | 0.423026 |
Target: 5'- aCCGGCcUCGGGGUUggCUGCCccUGCgGg -3' miRNA: 3'- -GGCCGuAGCUCUAGuaGGCGG--ACGgC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 16597 | 0.71 | 0.395797 |
Target: 5'- cUCGGUgcGUCGGGAaugccUCccugcgCCGCCUGCCa -3' miRNA: 3'- -GGCCG--UAGCUCU-----AGua----GGCGGACGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 44612 | 0.72 | 0.336833 |
Target: 5'- uCCGGCuugaucgaggCGAGGUCuUCgGCCUuGCCGu -3' miRNA: 3'- -GGCCGua--------GCUCUAGuAGgCGGA-CGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 27799 | 0.72 | 0.321205 |
Target: 5'- gCCGGcCGUCGAGGUaCAcgcgcUCCGUC-GCCGa -3' miRNA: 3'- -GGCC-GUAGCUCUA-GU-----AGGCGGaCGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 49550 | 0.72 | 0.306126 |
Target: 5'- aCGGCcgcGUCGGGGgcgucuUCGUaCCGCCUGCgCGg -3' miRNA: 3'- gGCCG---UAGCUCU------AGUA-GGCGGACG-GC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 1542 | 0.73 | 0.291594 |
Target: 5'- gCCGGCcgCcGGAUCGUCCGgCUUGUCu -3' miRNA: 3'- -GGCCGuaGcUCUAGUAGGC-GGACGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 33508 | 0.74 | 0.244992 |
Target: 5'- gCCGGCAUCGAuccgCAUCaCGgCUGCCu -3' miRNA: 3'- -GGCCGUAGCUcua-GUAG-GCgGACGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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