Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22735 | 5' | -52.1 | NC_005091.1 | + | 7172 | 0.66 | 0.892787 |
Target: 5'- gCCGgcAGCGGCGgc-CGUCUcGAUgCCCu -3' miRNA: 3'- aGGC--UCGCCGCauaGUAGAcUUA-GGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 30484 | 0.66 | 0.892787 |
Target: 5'- uUCgGGGCuGGCuucUCAUCUGAAUCa- -3' miRNA: 3'- -AGgCUCG-CCGcauAGUAGACUUAGgg -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 22811 | 0.66 | 0.891333 |
Target: 5'- gCCG-GCGGCGgaacgagCUGGaauGUCCCu -3' miRNA: 3'- aGGCuCGCCGCauagua-GACU---UAGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 54996 | 0.66 | 0.869857 |
Target: 5'- gCCGGGCGGCccgccgGUAUCccgugCUGAcgaCCCc -3' miRNA: 3'- aGGCUCGCCG------CAUAGua---GACUua-GGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 16865 | 0.67 | 0.852444 |
Target: 5'- gCCGAG-GGCGaucgcuaUAUCGUCgguaAGUCCCc -3' miRNA: 3'- aGGCUCgCCGC-------AUAGUAGac--UUAGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 43279 | 0.67 | 0.83579 |
Target: 5'- uUCC-AGCGGCGauUCgAUCUGGAUgCUa -3' miRNA: 3'- -AGGcUCGCCGCauAG-UAGACUUAgGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 6642 | 0.67 | 0.826703 |
Target: 5'- cCCGAGCGcGCGc-UCGUCUGcuUCgCu -3' miRNA: 3'- aGGCUCGC-CGCauAGUAGACuuAGgG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 7748 | 0.68 | 0.812683 |
Target: 5'- aCCGAGCGGCGaaauggagauGUCCCg -3' miRNA: 3'- aGGCUCGCCGCauaguagacuUAGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 10561 | 0.68 | 0.788365 |
Target: 5'- --aGAGUgaaauGGCGUAUCucgGUCUGAAucagcUCCCg -3' miRNA: 3'- aggCUCG-----CCGCAUAG---UAGACUU-----AGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 35069 | 0.68 | 0.788365 |
Target: 5'- cCCGuGCGGCGgcgGUCGUUccaaUGGccCCCu -3' miRNA: 3'- aGGCuCGCCGCa--UAGUAG----ACUuaGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 8722 | 0.68 | 0.778337 |
Target: 5'- aCCGAGaaugccgGGUGcaugAUCGcCUGAAUCCCc -3' miRNA: 3'- aGGCUCg------CCGCa---UAGUaGACUUAGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 48615 | 0.69 | 0.747369 |
Target: 5'- cUCgGGGCGGC----CGUCgGggUCCCa -3' miRNA: 3'- -AGgCUCGCCGcauaGUAGaCuuAGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 32524 | 0.71 | 0.637035 |
Target: 5'- gUCUGAGCcuGCGUAUCGcgaacuuUCUGGcuguccAUCCCa -3' miRNA: 3'- -AGGCUCGc-CGCAUAGU-------AGACU------UAGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 15602 | 0.72 | 0.560642 |
Target: 5'- aUCUGAGCGGCGgccCGUC-GAAUUUCa -3' miRNA: 3'- -AGGCUCGCCGCauaGUAGaCUUAGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 54836 | 0.73 | 0.517445 |
Target: 5'- gUCCGGGCGGCGccAUCAgcccaaAAUCCCc -3' miRNA: 3'- -AGGCUCGCCGCa-UAGUagac--UUAGGG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 12985 | 0.75 | 0.425877 |
Target: 5'- gCCGAGCGGCGUAUUGcCUGccgCCa -3' miRNA: 3'- aGGCUCGCCGCAUAGUaGACuuaGGg -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 35539 | 0.75 | 0.397547 |
Target: 5'- cUCCGAGCGGCGUA-CGgacaagggaUGggUCgCCa -3' miRNA: 3'- -AGGCUCGCCGCAUaGUag-------ACuuAG-GG- -5' |
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22735 | 5' | -52.1 | NC_005091.1 | + | 56238 | 1.12 | 0.001516 |
Target: 5'- gUCCGAGCGGCGUAUCAUCUGAAUCCCc -3' miRNA: 3'- -AGGCUCGCCGCAUAGUAGACUUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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