Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22736 | 3' | -54.1 | NC_005091.1 | + | 57380 | 0.66 | 0.793959 |
Target: 5'- aAGCgGGCGGCcaugaaauccucuggAAUUGCgCGGUCaUGCAg -3' miRNA: 3'- gUCG-CUGCCG---------------UUAACG-GCUAGcACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 56950 | 0.66 | 0.790008 |
Target: 5'- -uGCGAC-GCAGagcggGCCGGcCGUGCAc -3' miRNA: 3'- guCGCUGcCGUUaa---CGGCUaGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 56909 | 0.66 | 0.769863 |
Target: 5'- --aCGAuCGGCAAUUGCCG-UCGcuggcUGCGc -3' miRNA: 3'- gucGCU-GCCGUUAACGGCuAGC-----ACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 56829 | 1.09 | 0.001357 |
Target: 5'- cCAGCGACGGCAAUUGCCGAUCGUGCAg -3' miRNA: 3'- -GUCGCUGCCGUUAACGGCUAGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 55611 | 0.66 | 0.75853 |
Target: 5'- uCGGCGaagugauGCGGCAA--GCCGGgaugacgaCGUGCAa -3' miRNA: 3'- -GUCGC-------UGCCGUUaaCGGCUa-------GCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 54341 | 0.73 | 0.399325 |
Target: 5'- -cGCGAuCGGCAAcgcguuUUGCCcGUCGUGCu -3' miRNA: 3'- guCGCU-GCCGUU------AACGGcUAGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 54251 | 0.71 | 0.498279 |
Target: 5'- gUAGCacGACgGGCAaaacgcGUUGCCGAUCGcGCGg -3' miRNA: 3'- -GUCG--CUG-CCGU------UAACGGCUAGCaCGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 52794 | 0.7 | 0.540864 |
Target: 5'- -cGCGGCGGCGAUcagGCCGcacgCG-GCAg -3' miRNA: 3'- guCGCUGCCGUUAa--CGGCua--GCaCGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 51810 | 0.67 | 0.717168 |
Target: 5'- gGGCGuGCGGCuugcGCgCGAUUGUGUAc -3' miRNA: 3'- gUCGC-UGCCGuuaaCG-GCUAGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 49881 | 0.7 | 0.551708 |
Target: 5'- gCGGCGAucgcauCGGCAGaaGCCGAUCGaGCc -3' miRNA: 3'- -GUCGCU------GCCGUUaaCGGCUAGCaCGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 46540 | 0.66 | 0.769863 |
Target: 5'- -cGCGACGGC--UUGUCGAgCGUcGCc -3' miRNA: 3'- guCGCUGCCGuuAACGGCUaGCA-CGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 44875 | 0.72 | 0.418116 |
Target: 5'- gGGCGGCGGCAAggcgGCCGAagGcacgaagGCAa -3' miRNA: 3'- gUCGCUGCCGUUaa--CGGCUagCa------CGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 44605 | 0.69 | 0.570288 |
Target: 5'- aGGCGACGGCAGcgacgGCCuguaauaucgucucGA-CGUGCAg -3' miRNA: 3'- gUCGCUGCCGUUaa---CGG--------------CUaGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 41974 | 0.66 | 0.749138 |
Target: 5'- gUAGCGAagaGGacc-UGCCGA-CGUGCGg -3' miRNA: 3'- -GUCGCUg--CCguuaACGGCUaGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 37683 | 0.66 | 0.749138 |
Target: 5'- aCAuCGGCGGCAGgucUCGAUgGUGCAu -3' miRNA: 3'- -GUcGCUGCCGUUaacGGCUAgCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 36799 | 0.69 | 0.617854 |
Target: 5'- uGGCGACGGCGA--GCCGuUCGaccucGCGu -3' miRNA: 3'- gUCGCUGCCGUUaaCGGCuAGCa----CGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 34258 | 0.72 | 0.447285 |
Target: 5'- -cGCG-CGGC-AUUGCCGAuaUCGUGUc -3' miRNA: 3'- guCGCuGCCGuUAACGGCU--AGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 33329 | 0.67 | 0.738586 |
Target: 5'- gCAGCcgggucuuCGGCAA-UGCCGGUUGcUGCGu -3' miRNA: 3'- -GUCGcu------GCCGUUaACGGCUAGC-ACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 30884 | 0.68 | 0.644551 |
Target: 5'- aCAGCGACcaGGCGucgagGCCcaucgucagucgcguGAUCGUGCu -3' miRNA: 3'- -GUCGCUG--CCGUuaa--CGG---------------CUAGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 28459 | 0.81 | 0.120443 |
Target: 5'- gAGCGACGGCgAGUUGCCGAaguacaguucgccggUCGUGUu -3' miRNA: 3'- gUCGCUGCCG-UUAACGGCU---------------AGCACGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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