Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22737 | 3' | -60.5 | NC_005091.1 | + | 12697 | 0.69 | 0.298895 |
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Target: 5'- gCGGCUcuugUCCGUGAUgugUGCCGUCUGAUc -3' miRNA: 3'- -GUCGA----AGGCGCUGa--GCGGCGGGCUGa -5' |
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| 22737 | 3' | -60.5 | NC_005091.1 | + | 30187 | 0.7 | 0.27799 |
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Target: 5'- aAGCauggUCgGCGACuUCGCCGCCCu--- -3' miRNA: 3'- gUCGa---AGgCGCUG-AGCGGCGGGcuga -5' |
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| 22737 | 3' | -60.5 | NC_005091.1 | + | 19002 | 0.71 | 0.239701 |
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Target: 5'- cCAGCaagugaaUCCGCGACUgGCCGCgaugcUCGGCg -3' miRNA: 3'- -GUCGa------AGGCGCUGAgCGGCG-----GGCUGa -5' |
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| 22737 | 3' | -60.5 | NC_005091.1 | + | 6495 | 0.71 | 0.233765 |
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Target: 5'- -cGCaUCCGCauggUGCCGCCCGACUc -3' miRNA: 3'- guCGaAGGCGcugaGCGGCGGGCUGA- -5' |
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| 22737 | 3' | -60.5 | NC_005091.1 | + | 43061 | 0.71 | 0.222264 |
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Target: 5'- cCAGCUucucccgcgugUCUGCGACaggagccaaggUCGCCuuGCCCGGCg -3' miRNA: 3'- -GUCGA-----------AGGCGCUG-----------AGCGG--CGGGCUGa -5' |
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| 22737 | 3' | -60.5 | NC_005091.1 | + | 42344 | 0.72 | 0.180518 |
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Target: 5'- gUAGCccUCgGCGAgcgcuucCUCGCCGUCCGACa -3' miRNA: 3'- -GUCGa-AGgCGCU-------GAGCGGCGGGCUGa -5' |
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| 22737 | 3' | -60.5 | NC_005091.1 | + | 10342 | 0.73 | 0.176335 |
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Target: 5'- gAGUacUgCGCGugUCGCgCGCCCGACa -3' miRNA: 3'- gUCGa-AgGCGCugAGCG-GCGGGCUGa -5' |
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| 22737 | 3' | -60.5 | NC_005091.1 | + | 23260 | 0.78 | 0.074306 |
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Target: 5'- cCAGCUcgccgacgcacUgCGCGACaUGCCGCCCGGCUg -3' miRNA: 3'- -GUCGA-----------AgGCGCUGaGCGGCGGGCUGA- -5' |
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| 22737 | 3' | -60.5 | NC_005091.1 | + | 57134 | 1.07 | 0.000459 |
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Target: 5'- cCAGCUUCCGCGACUCGCCGCCCGACUg -3' miRNA: 3'- -GUCGAAGGCGCUGAGCGGCGGGCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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