Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22737 | 5' | -54.8 | NC_005091.1 | + | 57169 | 1.12 | 0.00071 |
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Target: 5'- uGGAAUGGGGCGGCGAAGACGCUGUUCa -3' miRNA: 3'- -CCUUACCCCGCCGCUUCUGCGACAAG- -5' |
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| 22737 | 5' | -54.8 | NC_005091.1 | + | 37432 | 0.69 | 0.572247 |
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Target: 5'- aGGaAGUGGcGaCGGCGAGGACGgaGUa- -3' miRNA: 3'- -CC-UUACCcC-GCCGCUUCUGCgaCAag -5' |
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| 22737 | 5' | -54.8 | NC_005091.1 | + | 16412 | 0.69 | 0.583131 |
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Target: 5'- ----gGGGGCGGCGGgauuccGGACGCa---- -3' miRNA: 3'- ccuuaCCCCGCCGCU------UCUGCGacaag -5' |
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| 22737 | 5' | -54.8 | NC_005091.1 | + | 29478 | 0.69 | 0.594056 |
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Target: 5'- cGAAccGGGCGaGCGAcguaGGACGCUGg-- -3' miRNA: 3'- cCUUacCCCGC-CGCU----UCUGCGACaag -5' |
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| 22737 | 5' | -54.8 | NC_005091.1 | + | 48966 | 0.68 | 0.605013 |
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Target: 5'- aGGAuUGGGGCuuGGCGGGcGCGCUa--- -3' miRNA: 3'- -CCUuACCCCG--CCGCUUcUGCGAcaag -5' |
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| 22737 | 5' | -54.8 | NC_005091.1 | + | 6024 | 0.68 | 0.659958 |
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Target: 5'- ----cGaGGGCGGCGcGGACGUUcgggcGUUCg -3' miRNA: 3'- ccuuaC-CCCGCCGCuUCUGCGA-----CAAG- -5' |
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| 22737 | 5' | -54.8 | NC_005091.1 | + | 34969 | 0.67 | 0.68944 |
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Target: 5'- cGGAAcagucggcuucgcuUGGcGGCGGCGGacuugaAGGCGCg--UCg -3' miRNA: 3'- -CCUU--------------ACC-CCGCCGCU------UCUGCGacaAG- -5' |
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| 22737 | 5' | -54.8 | NC_005091.1 | + | 24585 | 0.67 | 0.702428 |
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Target: 5'- ----cGGGGCGGCGucguugcAAGGCGCg---- -3' miRNA: 3'- ccuuaCCCCGCCGC-------UUCUGCGacaag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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