Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22738 | 3' | -60.9 | NC_005091.1 | + | 74 | 1.06 | 0.000492 |
Target: 5'- uCAAGGCGUGCGCCACGGCUGCCUCGUu -3' miRNA: 3'- -GUUCCGCACGCGGUGCCGACGGAGCA- -5' |
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22738 | 3' | -60.9 | NC_005091.1 | + | 11571 | 0.66 | 0.422131 |
Target: 5'- gCAGGGCaucgagcugGCGCUuCGGCUGUC-CGUa -3' miRNA: 3'- -GUUCCGca-------CGCGGuGCCGACGGaGCA- -5' |
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22738 | 3' | -60.9 | NC_005091.1 | + | 23464 | 0.69 | 0.257955 |
Target: 5'- --cGGCG-GCGCUgcguCGGCUGCCcCGg -3' miRNA: 3'- guuCCGCaCGCGGu---GCCGACGGaGCa -5' |
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22738 | 3' | -60.9 | NC_005091.1 | + | 24883 | 0.67 | 0.369257 |
Target: 5'- --cGGCGgccUGCGCagCACGGCggaauuccucgGCCUCGa -3' miRNA: 3'- guuCCGC---ACGCG--GUGCCGa----------CGGAGCa -5' |
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22738 | 3' | -60.9 | NC_005091.1 | + | 25101 | 0.67 | 0.360896 |
Target: 5'- gGAGGCG-GuCGCCAucgacgcgcCGGCUGCaUCGa -3' miRNA: 3'- gUUCCGCaC-GCGGU---------GCCGACGgAGCa -5' |
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22738 | 3' | -60.9 | NC_005091.1 | + | 26433 | 0.67 | 0.352669 |
Target: 5'- ---cGCGgGcCGCCGgGGCUGCCaUCGUa -3' miRNA: 3'- guucCGCaC-GCGGUgCCGACGG-AGCA- -5' |
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22738 | 3' | -60.9 | NC_005091.1 | + | 31595 | 0.67 | 0.369257 |
Target: 5'- --cGGCGUGC-CCGCgaaGGCUGCUuggCGUu -3' miRNA: 3'- guuCCGCACGcGGUG---CCGACGGa--GCA- -5' |
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22738 | 3' | -60.9 | NC_005091.1 | + | 33174 | 0.67 | 0.369257 |
Target: 5'- uCGAGcGCGUuaucgGcCGCCGCGaGCUGCUUCa- -3' miRNA: 3'- -GUUC-CGCA-----C-GCGGUGC-CGACGGAGca -5' |
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22738 | 3' | -60.9 | NC_005091.1 | + | 57468 | 1.06 | 0.000492 |
Target: 5'- uCAAGGCGUGCGCCACGGCUGCCUCGUu -3' miRNA: 3'- -GUUCCGCACGCGGUGCCGACGGAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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