miRNA: 3'- -GCUGCUCACGAAGUACCUCGUGCAGA- -5'
miRNA: 3'- gCUGCUcACGaAGUaCCUCGugCAGA- -5'
miRNA: 3'- gCUGCUCa-CGAAgU--ACC---UCGUGCAGa -5'
miRNA: 3'- -GCUGCUCACGAAGUACCUCGUGCAGA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22738 | 5' | -52.9 | NC_005091.1 | + | 109 | 1.09 | 0.001782 |
|
Target: 5'- aCGACGAGUGCUUCAUGGAGCACGUCUa -3' miRNA: 3'- -GCUGCUCACGAAGUACCUCGUGCAGA- -5' |
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| 22738 | 5' | -52.9 | NC_005091.1 | + | 20796 | 0.68 | 0.764716 |
|
Target: 5'- gGACGAuUGU-UCA-GGAGCugGUCUu -3' miRNA: 3'- gCUGCUcACGaAGUaCCUCGugCAGA- -5' |
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| 22738 | 5' | -52.9 | NC_005091.1 | + | 47321 | 0.67 | 0.794992 |
|
Target: 5'- uGAUGAGcgGCUUgAuuUGGuugAGCACGUCg -3' miRNA: 3'- gCUGCUCa-CGAAgU--ACC---UCGUGCAGa -5' |
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| 22738 | 5' | -52.9 | NC_005091.1 | + | 57503 | 1.09 | 0.001782 |
|
Target: 5'- aCGACGAGUGCUUCAUGGAGCACGUCUa -3' miRNA: 3'- -GCUGCUCACGAAGUACCUCGUGCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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