Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2275 | 3' | -50.6 | NC_001405.1 | + | 671 | 0.7 | 0.610163 |
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Target: 5'- cUCUUcGAUCAGCUGGuCCAAaaGACUgGCg -3' miRNA: 3'- -AGGAuUUGGUCGACC-GGUU--UUGGaCG- -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 8455 | 0.7 | 0.610163 |
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Target: 5'- cCCUGAcCCGGCUaugcgaGGU--AAACCUGCa -3' miRNA: 3'- aGGAUUuGGUCGA------CCGguUUUGGACG- -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 9927 | 0.69 | 0.645747 |
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Target: 5'- ---gAAGCCuggGGCaUGGCCGAccGCCUGCg -3' miRNA: 3'- aggaUUUGG---UCG-ACCGGUUu-UGGACG- -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 12493 | 0.69 | 0.645747 |
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Target: 5'- ---aGAGCCGGUUGGCCugcGgCUGCu -3' miRNA: 3'- aggaUUUGGUCGACCGGuuuUgGACG- -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 13193 | 0.68 | 0.704585 |
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Target: 5'- cUCCUGccCCAGCgcgcGGCUGAcACUUGUa -3' miRNA: 3'- -AGGAUuuGGUCGa---CCGGUUuUGGACG- -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 12611 | 0.68 | 0.716162 |
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Target: 5'- aCC-AGGCCGGCcucaucgGGCCGGAuggcCCUGUu -3' miRNA: 3'- aGGaUUUGGUCGa------CCGGUUUu---GGACG- -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 21381 | 0.66 | 0.803064 |
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Target: 5'- aUCCUGAGuuGuuauGCUGGUgAAgaauuccAACCUGCu -3' miRNA: 3'- -AGGAUUUggU----CGACCGgUU-------UUGGACG- -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 15169 | 0.66 | 0.81429 |
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Target: 5'- gUCCUccACCGGCUGcauuGCCGcgGCUgccGCg -3' miRNA: 3'- -AGGAuuUGGUCGAC----CGGUuuUGGa--CG- -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 23130 | 0.66 | 0.84349 |
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Target: 5'- ---aGAACCGGUggGGCCGAAAUgUGg -3' miRNA: 3'- aggaUUUGGUCGa-CCGGUUUUGgACg -5' |
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| 2275 | 3' | -50.6 | NC_001405.1 | + | 34861 | 0.76 | 0.277403 |
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Target: 5'- uUCCUGgcggggaaguGGCCGcGCUGGuCCGugcAGACCUGCa -3' miRNA: 3'- -AGGAU----------UUGGU-CGACC-GGU---UUUGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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