Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22757 | 5' | -42.7 | NC_005092.1 | + | 4154 | 0.66 | 0.925273 |
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Target: 5'- aCAUGuuGGGUcUAUCCCUGgcaUGACUa -3' miRNA: 3'- aGUGUggUCUAaAUAGGGAUa--AUUGG- -5' |
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| 22757 | 5' | -42.7 | NC_005092.1 | + | 3180 | 0.67 | 0.872145 |
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Target: 5'- cCACACCGGcaUUAUCCUccguggugggggAUUAACUg -3' miRNA: 3'- aGUGUGGUCuaAAUAGGGa-----------UAAUUGG- -5' |
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| 22757 | 5' | -42.7 | NC_005092.1 | + | 9015 | 0.68 | 0.853816 |
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Target: 5'- gCGCACCcaguuGAgaaUAUCCuccaCUAUUGGCCg -3' miRNA: 3'- aGUGUGGu----CUaa-AUAGG----GAUAAUUGG- -5' |
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| 22757 | 5' | -42.7 | NC_005092.1 | + | 2425 | 0.68 | 0.843119 |
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Target: 5'- uUCGCACCcaccuGGAUUcGUCCCaGUggaUGAUCa -3' miRNA: 3'- -AGUGUGG-----UCUAAaUAGGGaUA---AUUGG- -5' |
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| 22757 | 5' | -42.7 | NC_005092.1 | + | 8913 | 0.74 | 0.52979 |
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Target: 5'- -aACGCCA-AUUUAUCCCcAUUGAUCa -3' miRNA: 3'- agUGUGGUcUAAAUAGGGaUAAUUGG- -5' |
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| 22757 | 5' | -42.7 | NC_005092.1 | + | 4804 | 0.87 | 0.082534 |
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Target: 5'- cUCACACCAGAcuaacagGUCCaCUGUUAACCc -3' miRNA: 3'- -AGUGUGGUCUaaa----UAGG-GAUAAUUGG- -5' |
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| 22757 | 5' | -42.7 | NC_005092.1 | + | 8909 | 1.17 | 0.000744 |
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Target: 5'- gUCACACCAGAUUUAUCCCUAUUAACCa -3' miRNA: 3'- -AGUGUGGUCUAAAUAGGGAUAAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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