Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2277 | 5' | -49.2 | NC_001405.1 | + | 21563 | 0.66 | 0.917483 |
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Target: 5'- cGCUAGACaugaCUUUuGaGGUGGAUC-CCa -3' miRNA: 3'- cCGAUUUGg---GAAGuC-CCACUUAGaGG- -5' |
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| 2277 | 5' | -49.2 | NC_001405.1 | + | 6164 | 0.66 | 0.903651 |
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Target: 5'- cGGUgauGCCUUUgAGGGUGGccgCgUCCa -3' miRNA: 3'- -CCGauuUGGGAAgUCCCACUua-G-AGG- -5' |
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| 2277 | 5' | -49.2 | NC_001405.1 | + | 4191 | 0.67 | 0.888578 |
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Target: 5'- gGGCaUAAGCCCgucUCuGGGGUGGAgguagCaCCa -3' miRNA: 3'- -CCG-AUUUGGGa--AG-UCCCACUUa----GaGG- -5' |
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| 2277 | 5' | -49.2 | NC_001405.1 | + | 6047 | 0.67 | 0.872306 |
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Target: 5'- cGCUGucugcgagGGCCagcugUUGGGGUGAGUaCUCCc -3' miRNA: 3'- cCGAU--------UUGGga---AGUCCCACUUA-GAGG- -5' |
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| 2277 | 5' | -49.2 | NC_001405.1 | + | 16650 | 0.68 | 0.806855 |
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Target: 5'- gGGCUuguAAUCCUgcucuuccuucuUCGGGGggccauaGAUCUCCg -3' miRNA: 3'- -CCGAu--UUGGGA------------AGUCCCac-----UUAGAGG- -5' |
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| 2277 | 5' | -49.2 | NC_001405.1 | + | 34491 | 1.16 | 0.000935 |
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Target: 5'- aGGCUAAACCCUUCAGGGUGAAUCUCCu -3' miRNA: 3'- -CCGAUUUGGGAAGUCCCACUUAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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