miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22771 5' -56 NC_005093.1 + 1916 1.12 0.000053
Target:  5'- aCCAGGUCAAUGCGGAGAGAGCCCUCGg -3'
miRNA:   3'- -GGUCCAGUUACGCCUCUCUCGGGAGC- -5'
22771 5' -56 NC_005093.1 + 2605 0.66 0.227443
Target:  5'- aCAcuGUCAGUGUGGAGGGGGCgaCCaUCa -3'
miRNA:   3'- gGUc-CAGUUACGCCUCUCUCG--GG-AGc -5'
22771 5' -56 NC_005093.1 + 191 0.66 0.241103
Target:  5'- gCAGGacUCcgaggAGUGaagGGAG-GAGCCCUCGa -3'
miRNA:   3'- gGUCC--AG-----UUACg--CCUCuCUCGGGAGC- -5'
22771 5' -56 NC_005093.1 + 10528 0.66 0.248186
Target:  5'- uCCAuGUCAucUGCGGAGAGAacgagauucGUCUUCa -3'
miRNA:   3'- -GGUcCAGUu-ACGCCUCUCU---------CGGGAGc -5'
22771 5' -56 NC_005093.1 + 1926 0.69 0.158684
Target:  5'- uCCAGGUCcagggguuuGUGuCGGGGAcugGAGUUCUCGu -3'
miRNA:   3'- -GGUCCAGu--------UAC-GCCUCU---CUCGGGAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.