miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2280 5' -55.5 NC_001407.1 + 2284 0.71 0.090813
Target:  5'- cCGCgaaCCCGCAgauccauGGGAuaGGAGGGGGAa -3'
miRNA:   3'- -GUGag-GGGUGUau-----UCCU--CCUCCUCCU- -5'
2280 5' -55.5 NC_001407.1 + 3851 0.68 0.161798
Target:  5'- -cCUCCUCAagcaccCAUAAGGGGGugguagucuggaGGGAGGGc -3'
miRNA:   3'- guGAGGGGU------GUAUUCCUCC------------UCCUCCU- -5'
2280 5' -55.5 NC_001407.1 + 202 0.66 0.220182
Target:  5'- cCGCUCCCCGaAUAAGcGAgacGGAuGAGGGc -3'
miRNA:   3'- -GUGAGGGGUgUAUUC-CU---CCUcCUCCU- -5'
2280 5' -55.5 NC_001407.1 + 923 1.09 0.000069
Target:  5'- cCACUCCCCACAUAAGGAGGAGGAGGAg -3'
miRNA:   3'- -GUGAGGGGUGUAUUCCUCCUCCUCCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.