miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2282 3' -54.3 NC_001407.1 + 3803 0.67 0.182489
Target:  5'- uGAGGGUUaccgaccacccuGUGCCggGACCCACUGu -3'
miRNA:   3'- -UUCCCGAguu---------CAUGGa-CUGGGUGAUg -5'
2282 3' -54.3 NC_001407.1 + 8632 0.68 0.153493
Target:  5'- gGAGcGGCccaccuUUAAGUACCUGcagGCCCAgCUGCu -3'
miRNA:   3'- -UUC-CCG------AGUUCAUGGAC---UGGGU-GAUG- -5'
2282 3' -54.3 NC_001407.1 + 1044 0.74 0.049151
Target:  5'- cAGGGCcggccCAGGaGCCUGACCCGCaUGCc -3'
miRNA:   3'- uUCCCGa----GUUCaUGGACUGGGUG-AUG- -5'
2282 3' -54.3 NC_001407.1 + 3012 1.06 0.000115
Target:  5'- cAAGGGCUCAAGUACCUGACCCACUACc -3'
miRNA:   3'- -UUCCCGAGUUCAUGGACUGGGUGAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.