Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22820 | 3' | -47.8 | NC_005135.1 | + | 53244 | 0.66 | 0.999805 |
Target: 5'- ------aUAGGCGAuAGUCGCGAAAu -3' miRNA: 3'- acaauugGUCCGCUuUCGGCGUUUUc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 129215 | 0.66 | 0.999682 |
Target: 5'- cGUcGGCCuucaguuGaGCGAAAGCCGgGAAAa -3' miRNA: 3'- aCAaUUGGu------C-CGCUUUCGGCgUUUUc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 104385 | 0.66 | 0.999633 |
Target: 5'- aGUgGACCccgcugcuggaAGGCGAAGGCCugccggaaauugcugGCAAAAa -3' miRNA: 3'- aCAaUUGG-----------UCCGCUUUCGG---------------CGUUUUc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 168698 | 0.66 | 0.999598 |
Target: 5'- ---gAACCAGGUGA-AGCgGCAu--- -3' miRNA: 3'- acaaUUGGUCCGCUuUCGgCGUuuuc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 127896 | 0.66 | 0.999598 |
Target: 5'- ---gGGCCGGGUGccaaccuGAGUCGCGGAAc -3' miRNA: 3'- acaaUUGGUCCGCu------UUCGGCGUUUUc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 2973 | 0.66 | 0.999495 |
Target: 5'- aGUUAACgAgcgagcucgucGGCGAGGGCCGUu---- -3' miRNA: 3'- aCAAUUGgU-----------CCGCUUUCGGCGuuuuc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 125733 | 0.66 | 0.999484 |
Target: 5'- ---aAGCCAGGCGAgcgugaaGAGCgGUggauAAAAGg -3' miRNA: 3'- acaaUUGGUCCGCU-------UUCGgCG----UUUUC- -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 127996 | 0.67 | 0.99937 |
Target: 5'- -uUUAACCagauGGGCGAuAGCCGUc---- -3' miRNA: 3'- acAAUUGG----UCCGCUuUCGGCGuuuuc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 114992 | 0.67 | 0.99937 |
Target: 5'- aUGgac-CCGGGUGAGcugGGCCGUAAGGc -3' miRNA: 3'- -ACaauuGGUCCGCUU---UCGGCGUUUUc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 99779 | 0.67 | 0.999039 |
Target: 5'- --cUGGCCAcGGCGguAGCCGgCuGGAGa -3' miRNA: 3'- acaAUUGGU-CCGCuuUCGGC-GuUUUC- -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 93382 | 0.67 | 0.998569 |
Target: 5'- -aUUAGCC-GGCGAA-GCCGUucGAGc -3' miRNA: 3'- acAAUUGGuCCGCUUuCGGCGuuUUC- -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 99716 | 0.69 | 0.993407 |
Target: 5'- ---aAACCu-GCGAuaucAGCCGCAAGAGg -3' miRNA: 3'- acaaUUGGucCGCUu---UCGGCGUUUUC- -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 39719 | 0.7 | 0.99237 |
Target: 5'- ---gAAUCAGGUGAAuugaauGGCCGCAAc-- -3' miRNA: 3'- acaaUUGGUCCGCUU------UCGGCGUUuuc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 27109 | 0.7 | 0.99237 |
Target: 5'- --aUGGCCAGaGCGAAagcAGCUGCccaGAAAGa -3' miRNA: 3'- acaAUUGGUC-CGCUU---UCGGCG---UUUUC- -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 169047 | 0.7 | 0.98991 |
Target: 5'- uUGUUAGCgCAGGCGuugugAAGGCaCGCAc--- -3' miRNA: 3'- -ACAAUUG-GUCCGC-----UUUCG-GCGUuuuc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 36855 | 0.7 | 0.9867 |
Target: 5'- -aUUAGCCAGGCGAAaagcaguGGCCGa----- -3' miRNA: 3'- acAAUUGGUCCGCUU-------UCGGCguuuuc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 47419 | 0.71 | 0.985105 |
Target: 5'- gUGUUcaGACCguaaAGGUGAGAGCCGUg---- -3' miRNA: 3'- -ACAA--UUGG----UCCGCUUUCGGCGuuuuc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 133871 | 0.71 | 0.981044 |
Target: 5'- cUGUgaUGACCAuGCGAAAGCUGCcGGAu -3' miRNA: 3'- -ACA--AUUGGUcCGCUUUCGGCGuUUUc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 49360 | 0.72 | 0.967339 |
Target: 5'- cGUUGG-CAGGCGGAAGuUCGCGAAc- -3' miRNA: 3'- aCAAUUgGUCCGCUUUC-GGCGUUUuc -5' |
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22820 | 3' | -47.8 | NC_005135.1 | + | 157884 | 0.73 | 0.960262 |
Target: 5'- --aUGGCCAGGCGGAuGGCUGCu---- -3' miRNA: 3'- acaAUUGGUCCGCUU-UCGGCGuuuuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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