Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22821 | 3' | -50.9 | NC_005135.1 | + | 58002 | 0.66 | 0.993682 |
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Target: 5'- aGACC-CGGUuca-UCACcg-GGCCAGg -3' miRNA: 3'- -CUGGaGCCGuuuaAGUGauaCCGGUC- -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 12364 | 0.66 | 0.992598 |
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Target: 5'- aACCUUGGCucuugugaAAcgUCAUUgcaccgaGUGGCCGGa -3' miRNA: 3'- cUGGAGCCG--------UUuaAGUGA-------UACCGGUC- -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 129512 | 0.66 | 0.990382 |
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Target: 5'- uGCaCUUGGCAAuaaaAC-AUGGCCAGg -3' miRNA: 3'- cUG-GAGCCGUUuaagUGaUACCGGUC- -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 130642 | 0.66 | 0.990382 |
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Target: 5'- aACUUCGGCAAAgugUGCUGUgcaGGUCGGc -3' miRNA: 3'- cUGGAGCCGUUUaa-GUGAUA---CCGGUC- -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 22722 | 0.67 | 0.982042 |
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Target: 5'- uAUCUaCGGCAGAUUCugUGUG-CCGa -3' miRNA: 3'- cUGGA-GCCGUUUAAGugAUACcGGUc -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 157304 | 0.68 | 0.972158 |
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Target: 5'- gGAgCUCGGC-AAUUCAUcAUGGUgAGa -3' miRNA: 3'- -CUgGAGCCGuUUAAGUGaUACCGgUC- -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 18718 | 0.69 | 0.962523 |
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Target: 5'- cGCUUUGGCAAuUUCAUUcgAUGGCuCAGu -3' miRNA: 3'- cUGGAGCCGUUuAAGUGA--UACCG-GUC- -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 123481 | 0.72 | 0.886167 |
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Target: 5'- uGACCUCGGUAGAgugUCAUUccuuuugguauacgAaGGCCAGc -3' miRNA: 3'- -CUGGAGCCGUUUa--AGUGA--------------UaCCGGUC- -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 129104 | 0.72 | 0.866016 |
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Target: 5'- cGGCCUCGGCAAuaaaUCACUgaAUGGUa-- -3' miRNA: 3'- -CUGGAGCCGUUua--AGUGA--UACCGguc -5' |
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| 22821 | 3' | -50.9 | NC_005135.1 | + | 27128 | 1.1 | 0.00829 |
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Target: 5'- uGACCUCGGCAAAUUCACUAUGGCCAGa -3' miRNA: 3'- -CUGGAGCCGUUUAAGUGAUACCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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