Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22822 | 3' | -49.9 | NC_005135.1 | + | 81979 | 0.66 | 0.997181 |
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Target: 5'- uCGUCGCCaUGuuGUAUUGuGCCAGAUAa -3' miRNA: 3'- -GUAGUGG-GCgcCGUAAUuCGGUUUAU- -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 79517 | 0.66 | 0.99631 |
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Target: 5'- -uUgGCCCGUGGCGgaaaagaaaucaaaaUUGcgccuGGCCAAAUAg -3' miRNA: 3'- guAgUGGGCGCCGU---------------AAU-----UCGGUUUAU- -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 134861 | 0.66 | 0.995378 |
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Target: 5'- gGUC-CCCGUGGCuu---GCCGGAg- -3' miRNA: 3'- gUAGuGGGCGCCGuaauuCGGUUUau -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 47937 | 0.66 | 0.994602 |
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Target: 5'- gUAUCGCCUGaCGGUAUccgaugcuGGUCAGAUAu -3' miRNA: 3'- -GUAGUGGGC-GCCGUAau------UCGGUUUAU- -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 50686 | 0.67 | 0.988999 |
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Target: 5'- --aCACCgGCGGUGUUAAGCa----- -3' miRNA: 3'- guaGUGGgCGCCGUAAUUCGguuuau -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 19597 | 0.69 | 0.968698 |
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Target: 5'- -uUCGCCCGCcgGGUAUUcggaucacuGGCCAGGUGc -3' miRNA: 3'- guAGUGGGCG--CCGUAAu--------UCGGUUUAU- -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 103265 | 0.71 | 0.935426 |
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Target: 5'- -uUCAuCCCGCGGUcuc-GGCCAGGUGa -3' miRNA: 3'- guAGU-GGGCGCCGuaauUCGGUUUAU- -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 19905 | 0.77 | 0.67928 |
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Target: 5'- -uUCGCCUGCGGCGaagcgUUGAGCUAGAa- -3' miRNA: 3'- guAGUGGGCGCCGU-----AAUUCGGUUUau -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 21419 | 0.77 | 0.672859 |
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Target: 5'- cCAUCAagaaaauacuggcauCCCGCGGCGUUAAGCUcGAUc -3' miRNA: 3'- -GUAGU---------------GGGCGCCGUAAUUCGGuUUAu -5' |
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| 22822 | 3' | -49.9 | NC_005135.1 | + | 27630 | 1.08 | 0.012023 |
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Target: 5'- aCAUCACCCGCGGCAUUAAGCCAAAUAa -3' miRNA: 3'- -GUAGUGGGCGCCGUAAUUCGGUUUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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