Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22825 | 5' | -44.3 | NC_005135.1 | + | 15603 | 0.66 | 0.999999 |
Target: 5'- aACAauUGGCU---GACGCCUACAAa-- -3' miRNA: 3'- cUGU--GCCGAuuaUUGUGGAUGUUaaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 74610 | 0.66 | 0.999999 |
Target: 5'- cGGCACcuucugcaGGCUGA--ACACCaACGAUUUg -3' miRNA: 3'- -CUGUG--------CCGAUUauUGUGGaUGUUAAA- -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 168814 | 0.66 | 0.999998 |
Target: 5'- -cCACGGUUGGUcucuugguCACCUACGAa-- -3' miRNA: 3'- cuGUGCCGAUUAuu------GUGGAUGUUaaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 105410 | 0.67 | 0.999997 |
Target: 5'- -gUugGGCUGGUGAauCCUACAAa-- -3' miRNA: 3'- cuGugCCGAUUAUUguGGAUGUUaaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 724 | 0.67 | 0.999995 |
Target: 5'- cAUACGGU----GACACCUACGAc-- -3' miRNA: 3'- cUGUGCCGauuaUUGUGGAUGUUaaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 55038 | 0.67 | 0.999993 |
Target: 5'- aAUACGGaUGAUAccuuCACCUACAAUg- -3' miRNA: 3'- cUGUGCCgAUUAUu---GUGGAUGUUAaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 156843 | 0.68 | 0.999973 |
Target: 5'- uGACgACGGCgauuuguAUACCUACGAUa- -3' miRNA: 3'- -CUG-UGCCGauuau--UGUGGAUGUUAaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 153237 | 0.68 | 0.999963 |
Target: 5'- aGCGCGuGC----AACGCCUGCAGUUa -3' miRNA: 3'- cUGUGC-CGauuaUUGUGGAUGUUAAa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 107627 | 0.7 | 0.999456 |
Target: 5'- aGCACGGCUAAUGguAUGCCgucACGGUa- -3' miRNA: 3'- cUGUGCCGAUUAU--UGUGGa--UGUUAaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 98366 | 0.71 | 0.998708 |
Target: 5'- cGGCACGGCcAGUGGCugACCgaacgACAGUUg -3' miRNA: 3'- -CUGUGCCGaUUAUUG--UGGa----UGUUAAa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 72290 | 0.72 | 0.998421 |
Target: 5'- aGAUaaAUGGCUAGUGgcagccaaGCGCCUGCGAUc- -3' miRNA: 3'- -CUG--UGCCGAUUAU--------UGUGGAUGUUAaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 8217 | 0.72 | 0.998082 |
Target: 5'- cACGCaGUUGAUGACAUCUGCAGc-- -3' miRNA: 3'- cUGUGcCGAUUAUUGUGGAUGUUaaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 149372 | 0.75 | 0.980838 |
Target: 5'- uGAUACGGUUGAagauauUGGCGCCUGCGGa-- -3' miRNA: 3'- -CUGUGCCGAUU------AUUGUGGAUGUUaaa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 81795 | 0.77 | 0.94568 |
Target: 5'- aACAUGGCgacGAUAACACCUGCuGUUa -3' miRNA: 3'- cUGUGCCGa--UUAUUGUGGAUGuUAAa -5' |
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22825 | 5' | -44.3 | NC_005135.1 | + | 102753 | 0.95 | 0.229023 |
Target: 5'- cGACACGGCUAAUAACACCU-CAAUUUc -3' miRNA: 3'- -CUGUGCCGAUUAUUGUGGAuGUUAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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