Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22826 | 5' | -45.8 | NC_005135.1 | + | 162909 | 0.66 | 0.999989 |
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Target: 5'- aUgGCGuuGGCAgcaACCGAGAACAc--- -3' miRNA: 3'- cAgCGUuuCUGU---UGGCUCUUGUauaa -5' |
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| 22826 | 5' | -45.8 | NC_005135.1 | + | 91983 | 0.66 | 0.999979 |
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Target: 5'- gGUCGCAGuuGGCAACCG-GAAUc---- -3' miRNA: 3'- -CAGCGUUu-CUGUUGGCuCUUGuauaa -5' |
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| 22826 | 5' | -45.8 | NC_005135.1 | + | 145633 | 0.66 | 0.99997 |
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Target: 5'- aUCGCAGuuGCAACCGAGuucACGa--- -3' miRNA: 3'- cAGCGUUucUGUUGGCUCu--UGUauaa -5' |
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| 22826 | 5' | -45.8 | NC_005135.1 | + | 49547 | 0.67 | 0.99996 |
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Target: 5'- aUUGC--AGACAGCCGAaAACGUAg- -3' miRNA: 3'- cAGCGuuUCUGUUGGCUcUUGUAUaa -5' |
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| 22826 | 5' | -45.8 | NC_005135.1 | + | 47050 | 0.67 | 0.999927 |
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Target: 5'- uGUCGCAAAG-CGGCCagucGAGAACc---- -3' miRNA: 3'- -CAGCGUUUCuGUUGG----CUCUUGuauaa -5' |
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| 22826 | 5' | -45.8 | NC_005135.1 | + | 118171 | 0.67 | 0.999904 |
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Target: 5'- uUCGCAAAuuCGGCCagacuguugagaGAGAACAUAUa -3' miRNA: 3'- cAGCGUUUcuGUUGG------------CUCUUGUAUAa -5' |
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| 22826 | 5' | -45.8 | NC_005135.1 | + | 11429 | 0.67 | 0.999873 |
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Target: 5'- uGUCGuCAccagucGGGACuuccucgaGGCCGAGAGCAUAa- -3' miRNA: 3'- -CAGC-GU------UUCUG--------UUGGCUCUUGUAUaa -5' |
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| 22826 | 5' | -45.8 | NC_005135.1 | + | 146171 | 0.7 | 0.997232 |
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Target: 5'- uGUUGCuauAGGCGACCGAGuACAa--- -3' miRNA: 3'- -CAGCGuu-UCUGUUGGCUCuUGUauaa -5' |
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| 22826 | 5' | -45.8 | NC_005135.1 | + | 122285 | 1.07 | 0.039141 |
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Target: 5'- gGUCGCAAAGACAACCGAGAACAUAUUg -3' miRNA: 3'- -CAGCGUUUCUGUUGGCUCUUGUAUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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