miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22827 5' -48.6 NC_005135.1 + 87348 0.66 0.999517
Target:  5'- gCGCCGGCGauaacguUUAUGCUgaGGUCA-AGUCc -3'
miRNA:   3'- -GCGGUUGC-------AGUACGA--CCAGUuUUAGc -5'
22827 5' -48.6 NC_005135.1 + 105474 0.67 0.999105
Target:  5'- uCGCCAAacuggcCGUgGUGCUGG-CAAcuUCa -3'
miRNA:   3'- -GCGGUU------GCAgUACGACCaGUUuuAGc -5'
22827 5' -48.6 NC_005135.1 + 47934 0.67 0.998907
Target:  5'- uCGCCuGACGguauccgAUGCUGGUCAGAu--- -3'
miRNA:   3'- -GCGG-UUGCag-----UACGACCAGUUUuagc -5'
22827 5' -48.6 NC_005135.1 + 164365 0.67 0.998395
Target:  5'- uCGUUAugGcCggGCUGGUCAucgguuggaagAAAUCGg -3'
miRNA:   3'- -GCGGUugCaGuaCGACCAGU-----------UUUAGC- -5'
22827 5' -48.6 NC_005135.1 + 172510 0.68 0.997694
Target:  5'- aCGCCAaaauGCGUUgcGCUGGUUauGAAGUUc -3'
miRNA:   3'- -GCGGU----UGCAGuaCGACCAG--UUUUAGc -5'
22827 5' -48.6 NC_005135.1 + 140817 0.68 0.996753
Target:  5'- uCGCCGagaugaauagcaACuUCAUGCUGGUCAcgg-CGa -3'
miRNA:   3'- -GCGGU------------UGcAGUACGACCAGUuuuaGC- -5'
22827 5' -48.6 NC_005135.1 + 138527 0.69 0.994768
Target:  5'- gCGUCGACGUCggGUUGGgucucccauUCAAuAUCGc -3'
miRNA:   3'- -GCGGUUGCAGuaCGACC---------AGUUuUAGC- -5'
22827 5' -48.6 NC_005135.1 + 35358 0.7 0.984482
Target:  5'- aGCCGAUGa---GCUGGUCAAAAUgCGu -3'
miRNA:   3'- gCGGUUGCaguaCGACCAGUUUUA-GC- -5'
22827 5' -48.6 NC_005135.1 + 104582 0.71 0.972778
Target:  5'- -aCCAACaUCGcUGCUGGUCAAAGUUc -3'
miRNA:   3'- gcGGUUGcAGU-ACGACCAGUUUUAGc -5'
22827 5' -48.6 NC_005135.1 + 9294 0.79 0.729284
Target:  5'- gCGCCGACGgcauUCAUGCUGGUguAAAguUCGu -3'
miRNA:   3'- -GCGGUUGC----AGUACGACCAguUUU--AGC- -5'
22827 5' -48.6 NC_005135.1 + 155280 1.09 0.01725
Target:  5'- uCGCCAACGUCAUGCUGGUCAAAAUCGc -3'
miRNA:   3'- -GCGGUUGCAGUACGACCAGUUUUAGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.