Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22828 | 3' | -50.2 | NC_005135.1 | + | 164361 | 0.66 | 0.99315 |
Target: 5'- uAugGC-CGGGCUGGUc-AUCGGUUg- -3' miRNA: 3'- -UugCGaGUCCGACCAacUAGCUAAau -5' |
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22828 | 3' | -50.2 | NC_005135.1 | + | 33757 | 0.66 | 0.992086 |
Target: 5'- aAACGCUCGGGUUcaaGGUUGAaUGGa--- -3' miRNA: 3'- -UUGCGAGUCCGA---CCAACUaGCUaaau -5' |
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22828 | 3' | -50.2 | NC_005135.1 | + | 18699 | 0.69 | 0.963188 |
Target: 5'- gAugGCUCA-GUUGGUcaUGAUUGGUUUGa -3' miRNA: 3'- -UugCGAGUcCGACCA--ACUAGCUAAAU- -5' |
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22828 | 3' | -50.2 | NC_005135.1 | + | 8263 | 1.05 | 0.016 |
Target: 5'- cAACGCUCAGGCUGGUUGAUCGAUUUAg -3' miRNA: 3'- -UUGCGAGUCCGACCAACUAGCUAAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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