Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22828 | 5' | -50.7 | NC_005135.1 | + | 19820 | 0.66 | 0.99479 |
Target: 5'- uGCCAACgUUCUCAugCAcCUgGCCa- -3' miRNA: 3'- -CGGUUGaAGGAGUugGUuGAaCGGgu -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 39113 | 0.66 | 0.994298 |
Target: 5'- -aUAGCUUCCggCGGCCAuaucaguuacguugaGC-UGCCCAa -3' miRNA: 3'- cgGUUGAAGGa-GUUGGU---------------UGaACGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 51638 | 0.66 | 0.99395 |
Target: 5'- aCCGGCUUCgUUAAaCGACUUGCUgAg -3' miRNA: 3'- cGGUUGAAGgAGUUgGUUGAACGGgU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 61795 | 0.66 | 0.99395 |
Target: 5'- uGUUcGCUUCCUCGACuCAGUUUGUUCAg -3' miRNA: 3'- -CGGuUGAAGGAGUUG-GUUGAACGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 160955 | 0.66 | 0.99395 |
Target: 5'- aGCgAAUcauacuggUUCCUCAACCAACUccguggcauggGUCCAu -3' miRNA: 3'- -CGgUUG--------AAGGAGUUGGUUGAa----------CGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 138262 | 0.66 | 0.993005 |
Target: 5'- cGCCGACUUCaa-GACCGcAUaUGCCUg -3' miRNA: 3'- -CGGUUGAAGgagUUGGU-UGaACGGGu -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 146659 | 0.67 | 0.990762 |
Target: 5'- cGUCAACccgUUCaCUUuugGAgCAACUUGCCCGa -3' miRNA: 3'- -CGGUUG---AAG-GAG---UUgGUUGAACGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 59842 | 0.68 | 0.980557 |
Target: 5'- cCCgAAUUUCCUUAAUCAGCg-GCCCu -3' miRNA: 3'- cGG-UUGAAGGAGUUGGUUGaaCGGGu -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 112750 | 0.69 | 0.970133 |
Target: 5'- cGCCAACaUCCUgAACCAcggGCUuUGUUCGa -3' miRNA: 3'- -CGGUUGaAGGAgUUGGU---UGA-ACGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 51422 | 0.69 | 0.970133 |
Target: 5'- -gCAACUUCUUCAGCCAGCUcaGCa-- -3' miRNA: 3'- cgGUUGAAGGAGUUGGUUGAa-CGggu -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 65176 | 0.69 | 0.963639 |
Target: 5'- cGCCAACUUUCUUuuucgGACCAguGCUauCCCAc -3' miRNA: 3'- -CGGUUGAAGGAG-----UUGGU--UGAacGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 86852 | 0.69 | 0.963639 |
Target: 5'- -aCAACUUCCggugCAcugACCAugACUcUGCCCAa -3' miRNA: 3'- cgGUUGAAGGa---GU---UGGU--UGA-ACGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 164153 | 0.69 | 0.960047 |
Target: 5'- cCCGAUUUCUUcCAACCGAUgaccaGCCCGg -3' miRNA: 3'- cGGUUGAAGGA-GUUGGUUGaa---CGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 152707 | 0.7 | 0.94326 |
Target: 5'- cGCCGGCUcggCCauUUAACCA--UUGCCCAu -3' miRNA: 3'- -CGGUUGAa--GG--AGUUGGUugAACGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 35026 | 0.73 | 0.836162 |
Target: 5'- aGCCAGCUcgacuacaUCaUCAGCgCAGCUaaUGCCCAg -3' miRNA: 3'- -CGGUUGA--------AGgAGUUG-GUUGA--ACGGGU- -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 27235 | 0.73 | 0.836162 |
Target: 5'- uCCAGCaaaUCUUCAACCAACUUGCg-- -3' miRNA: 3'- cGGUUGa--AGGAGUUGGUUGAACGggu -5' |
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22828 | 5' | -50.7 | NC_005135.1 | + | 8297 | 1.12 | 0.006517 |
Target: 5'- cGCCAACUUCCUCAACCAACUUGCCCAu -3' miRNA: 3'- -CGGUUGAAGGAGUUGGUUGAACGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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