Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22829 | 3' | -49.5 | NC_005135.1 | + | 169849 | 0.66 | 0.997807 |
Target: 5'- cGCUAAcaaacGGGUACCAauggcugcUGCAGAUguauugGCCGu -3' miRNA: 3'- -CGGUU-----UCCGUGGU--------ACGUUUAa-----CGGUg -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 81314 | 0.66 | 0.997807 |
Target: 5'- --uGAAGGUgaaACaucaGUGCAAAUUGCCGg -3' miRNA: 3'- cggUUUCCG---UGg---UACGUUUAACGGUg -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 130647 | 0.66 | 0.996889 |
Target: 5'- cGgCAAAGuGUGCUGUGCAGGUcgGCCGu -3' miRNA: 3'- -CgGUUUC-CGUGGUACGUUUAa-CGGUg -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 149028 | 0.67 | 0.994934 |
Target: 5'- gGCCcGAGGUggGCCAUGaauuGAGUcGCCAUu -3' miRNA: 3'- -CGGuUUCCG--UGGUACg---UUUAaCGGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 45415 | 0.67 | 0.994934 |
Target: 5'- aGCaCGAAcGGCGCCGauuCAAcgauAUUGCCGCa -3' miRNA: 3'- -CG-GUUU-CCGUGGUac-GUU----UAACGGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 103893 | 0.67 | 0.994096 |
Target: 5'- aGCCAGuuGGGUuUC-UGCAGAgcUUGCCAUa -3' miRNA: 3'- -CGGUU--UCCGuGGuACGUUU--AACGGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 52413 | 0.68 | 0.990895 |
Target: 5'- aCCAGAGGCuuAUCAUGCAuuuAUcGCgGCu -3' miRNA: 3'- cGGUUUCCG--UGGUACGUu--UAaCGgUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 39381 | 0.68 | 0.986461 |
Target: 5'- aCCAAGGGCAaggaaauuUGCugaagauGUUGCCGCg -3' miRNA: 3'- cGGUUUCCGUggu-----ACGuu-----UAACGGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 165883 | 0.68 | 0.986461 |
Target: 5'- cGgCAuu-GCACCGagGCGAAUUGCCAUc -3' miRNA: 3'- -CgGUuucCGUGGUa-CGUUUAACGGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 72532 | 0.68 | 0.986461 |
Target: 5'- cCCGAAGcuuguGCGCCGUGCAGGUguaUGgCAUg -3' miRNA: 3'- cGGUUUC-----CGUGGUACGUUUA---ACgGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 172907 | 0.69 | 0.978178 |
Target: 5'- cGCCAGAGGCAU--UGUAAGUcucagaGCCAa -3' miRNA: 3'- -CGGUUUCCGUGguACGUUUAa-----CGGUg -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 72308 | 0.69 | 0.978178 |
Target: 5'- aGCCAA--GCGCC-UGCGAucaUGCCAUa -3' miRNA: 3'- -CGGUUucCGUGGuACGUUua-ACGGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 119807 | 0.69 | 0.975621 |
Target: 5'- uGCCuuuGAGGCGCUcgGCAAAcucacucaauUUGCUg- -3' miRNA: 3'- -CGGu--UUCCGUGGuaCGUUU----------AACGGug -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 26802 | 0.69 | 0.975621 |
Target: 5'- uGCCAucGaGCGCC-UGCAAGcUGCuCGCc -3' miRNA: 3'- -CGGUuuC-CGUGGuACGUUUaACG-GUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 104484 | 0.7 | 0.96986 |
Target: 5'- gGCCAGguAGGCgGCCAUGgGAucauUUGCCuGCu -3' miRNA: 3'- -CGGUU--UCCG-UGGUACgUUu---AACGG-UG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 164005 | 0.7 | 0.966642 |
Target: 5'- uUCAAAGGCACCGauUGCAAuacccAUUGCg-- -3' miRNA: 3'- cGGUUUCCGUGGU--ACGUU-----UAACGgug -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 98404 | 0.7 | 0.963188 |
Target: 5'- cUCAuAGGCAggaccCCAUGCGAAUuUGCCGa -3' miRNA: 3'- cGGUuUCCGU-----GGUACGUUUA-ACGGUg -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 61248 | 0.71 | 0.951353 |
Target: 5'- aCCAAGGGUucgacACCuUGCGAAacGCCACc -3' miRNA: 3'- cGGUUUCCG-----UGGuACGUUUaaCGGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 69765 | 0.71 | 0.9469 |
Target: 5'- uGCCAGAGGCGa---GCGGGUUacaGCCGCu -3' miRNA: 3'- -CGGUUUCCGUgguaCGUUUAA---CGGUG- -5' |
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22829 | 3' | -49.5 | NC_005135.1 | + | 115260 | 0.72 | 0.92475 |
Target: 5'- uGCCAcAAGGUACUacucucugcccagcGUGCAAAaUGCCAg -3' miRNA: 3'- -CGGU-UUCCGUGG--------------UACGUUUaACGGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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