Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22830 | 5' | -48.7 | NC_005135.1 | + | 105674 | 0.67 | 0.997375 |
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Target: 5'- -----gUUGCCAGcaccacGGC-CAGUUUGGCg -3' miRNA: 3'- aagguaAACGGUU------UCGcGUCAAACCG- -5' |
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| 22830 | 5' | -48.7 | NC_005135.1 | + | 105275 | 0.67 | 0.994891 |
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Target: 5'- -aCCAUUgaaGCCuu-GaCGCAGUUcGGCg -3' miRNA: 3'- aaGGUAAa--CGGuuuC-GCGUCAAaCCG- -5' |
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| 22830 | 5' | -48.7 | NC_005135.1 | + | 75354 | 0.68 | 0.994038 |
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Target: 5'- -aUCGUUuaauggguucUGCCAAAGCGUA-UUUGGUa -3' miRNA: 3'- aaGGUAA----------ACGGUUUCGCGUcAAACCG- -5' |
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| 22830 | 5' | -48.7 | NC_005135.1 | + | 1149 | 0.71 | 0.950229 |
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Target: 5'- cUUCCAUgaucgacaGCCAAAGCGUc-UUUGGCc -3' miRNA: 3'- -AAGGUAaa------CGGUUUCGCGucAAACCG- -5' |
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| 22830 | 5' | -48.7 | NC_005135.1 | + | 95217 | 0.72 | 0.945659 |
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Target: 5'- aUCCugaaaauaugGUUUGCCAucGCGCuGUcgGGCc -3' miRNA: 3'- aAGG----------UAAACGGUuuCGCGuCAaaCCG- -5' |
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| 22830 | 5' | -48.7 | NC_005135.1 | + | 86729 | 0.72 | 0.924685 |
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Target: 5'- cUUCgGUUUGauCCAAAGCGCGGguuaUUGGUu -3' miRNA: 3'- -AAGgUAAAC--GGUUUCGCGUCa---AACCG- -5' |
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| 22830 | 5' | -48.7 | NC_005135.1 | + | 18151 | 0.74 | 0.865054 |
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Target: 5'- gUUCGUUaagGCCAuugcagaagaauuGCGCGGUUUGGCa -3' miRNA: 3'- aAGGUAAa--CGGUuu-----------CGCGUCAAACCG- -5' |
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| 22830 | 5' | -48.7 | NC_005135.1 | + | 16288 | 1.1 | 0.01054 |
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Target: 5'- uUUCCAUUUGCCAAAGCGCAGUUUGGCu -3' miRNA: 3'- -AAGGUAAACGGUUUCGCGUCAAACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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