Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22833 | 3' | -42.8 | NC_005135.1 | + | 109366 | 0.67 | 1 |
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Target: 5'- --uAAUUACUCC---GCAGGUUUGGUu -3' miRNA: 3'- uauUUAAUGAGGaacCGUCUAAACUA- -5' |
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| 22833 | 3' | -42.8 | NC_005135.1 | + | 169539 | 0.66 | 1 |
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Target: 5'- cAUAAcUUAaUCCUUGGCGGAgg-GAc -3' miRNA: 3'- -UAUUuAAUgAGGAACCGUCUaaaCUa -5' |
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| 22833 | 3' | -42.8 | NC_005135.1 | + | 170555 | 0.66 | 1 |
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Target: 5'- ------cACUCUUUcGGCAGAUUUGc- -3' miRNA: 3'- uauuuaaUGAGGAA-CCGUCUAAACua -5' |
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| 22833 | 3' | -42.8 | NC_005135.1 | + | 110785 | 0.9 | 0.504087 |
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Target: 5'- cAUGAuaUGCUCCUUGGUAGAUUUGAUg -3' miRNA: 3'- -UAUUuaAUGAGGAACCGUCUAAACUA- -5' |
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| 22833 | 3' | -42.8 | NC_005135.1 | + | 44506 | 1 | 0.171899 |
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Target: 5'- uUAAAUUACUCCUUGGUAGAUUUGAUg -3' miRNA: 3'- uAUUUAAUGAGGAACCGUCUAAACUA- -5' |
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| 22833 | 3' | -42.8 | NC_005135.1 | + | 72168 | 1 | 0.171899 |
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Target: 5'- uUAAAUUACUCCUUGGUAGAUUUGAUg -3' miRNA: 3'- uAUUUAAUGAGGAACCGUCUAAACUA- -5' |
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| 22833 | 3' | -42.8 | NC_005135.1 | + | 54253 | 1.06 | 0.080255 |
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Target: 5'- cAUAAAUUACUCCUUGGCAGAUUUGAUg -3' miRNA: 3'- -UAUUUAAUGAGGAACCGUCUAAACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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