Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22833 | 5' | -45.4 | NC_005135.1 | + | 166113 | 0.66 | 0.999996 |
Target: 5'- uGGCCAUAGUCaugaCGUUGUaUGgcAGCa -3' miRNA: 3'- uUUGGUAUCAGg---GUAGCA-ACauUUGa -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 165014 | 0.66 | 0.999988 |
Target: 5'- --cCUAUAG-CaCUAUCGUUGUAAACc -3' miRNA: 3'- uuuGGUAUCaG-GGUAGCAACAUUUGa -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 46642 | 0.67 | 0.999977 |
Target: 5'- -uACUAUAGUCCCAUCaaaucAAGCUa -3' miRNA: 3'- uuUGGUAUCAGGGUAGcaacaUUUGA- -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 59252 | 0.68 | 0.999771 |
Target: 5'- --uCCAUAGcCUCAUaGUUGUAAACa -3' miRNA: 3'- uuuGGUAUCaGGGUAgCAACAUUUGa -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 169570 | 0.71 | 0.997973 |
Target: 5'- uAAACCAUAuUCCCAUCagcGUAAACa -3' miRNA: 3'- -UUUGGUAUcAGGGUAGcaaCAUUUGa -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 110825 | 0.77 | 0.93601 |
Target: 5'- --uCCAUAGUCCCAcCaUUGUAAACUa -3' miRNA: 3'- uuuGGUAUCAGGGUaGcAACAUUUGA- -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 159268 | 0.79 | 0.869174 |
Target: 5'- gAGGCUAUAGUauccuagagcCUCAUCGUUGUAAACUa -3' miRNA: 3'- -UUUGGUAUCA----------GGGUAGCAACAUUUGA- -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 46471 | 0.85 | 0.594088 |
Target: 5'- --uCCAUAGUCCCAUCaUUGUAAACa -3' miRNA: 3'- uuuGGUAUCAGGGUAGcAACAUUUGa -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 44545 | 0.87 | 0.497154 |
Target: 5'- --uCCAUAGUCCCAUCaUUGUAAACUa -3' miRNA: 3'- uuuGGUAUCAGGGUAGcAACAUUUGA- -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 72204 | 0.92 | 0.269536 |
Target: 5'- uAAACCAUAGUCCCAUCaUUGUAAACa -3' miRNA: 3'- -UUUGGUAUCAGGGUAGcAACAUUUGa -5' |
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22833 | 5' | -45.4 | NC_005135.1 | + | 54290 | 1.07 | 0.042082 |
Target: 5'- uAAACCAUAGUCCCAUCGUUGUAAACUa -3' miRNA: 3'- -UUUGGUAUCAGGGUAGCAACAUUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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