Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22836 | 5' | -53.8 | NC_005135.1 | + | 129831 | 0.66 | 0.96881 |
Target: 5'- uAUCGUGCUuuacuuCACgUGCAuuuuCGGUGCg -3' miRNA: 3'- cUAGUACGGuau---GUGgACGU----GCCGCG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 73731 | 0.66 | 0.962271 |
Target: 5'- -uUCAUGaacCCAaACugCUGgACGGCGg -3' miRNA: 3'- cuAGUAC---GGUaUGugGACgUGCCGCg -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 28677 | 0.66 | 0.96192 |
Target: 5'- gGGUUAUGCCAUuagcuuucgaauaAUcgaugGCCUGCACGaaCGCa -3' miRNA: 3'- -CUAGUACGGUA-------------UG-----UGGACGUGCc-GCG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 164193 | 0.67 | 0.950768 |
Target: 5'- aAUCgGUGCCuuugaAUugACCggUGCACuGGCGCu -3' miRNA: 3'- cUAG-UACGG-----UAugUGG--ACGUG-CCGCG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 34173 | 0.67 | 0.943766 |
Target: 5'- -uUCAuUGCCguacguaauuuuaccGUccACACCUGCGCGGC-Ca -3' miRNA: 3'- cuAGU-ACGG---------------UA--UGUGGACGUGCCGcG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 43230 | 0.67 | 0.937132 |
Target: 5'- -----cGCgAaGCAUCUGUAUGGCGCu -3' miRNA: 3'- cuaguaCGgUaUGUGGACGUGCCGCG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 68153 | 0.67 | 0.937132 |
Target: 5'- -uUCAgUGCCAUGCugCaGCaACGGCu- -3' miRNA: 3'- cuAGU-ACGGUAUGugGaCG-UGCCGcg -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 91393 | 0.67 | 0.937132 |
Target: 5'- gGAUUAagaaCGgagaGCGCCUGUGCGGCGCc -3' miRNA: 3'- -CUAGUacg-GUa---UGUGGACGUGCCGCG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 76662 | 0.68 | 0.926821 |
Target: 5'- cGAUUuguUGUUAUuugcgGCACCUGaaucgGCGGCGCg -3' miRNA: 3'- -CUAGu--ACGGUA-----UGUGGACg----UGCCGCG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 165089 | 0.68 | 0.926279 |
Target: 5'- cGAUCGUauggaggGCCAUGcCAUCUGCAgGGUccaccGCg -3' miRNA: 3'- -CUAGUA-------CGGUAU-GUGGACGUgCCG-----CG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 1613 | 0.68 | 0.903253 |
Target: 5'- cGGUCGUucugGCCAaGCAUUUGCuauuauugGCGGCGUa -3' miRNA: 3'- -CUAGUA----CGGUaUGUGGACG--------UGCCGCG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 27599 | 0.69 | 0.860788 |
Target: 5'- -----cGCCAU-CACUUGUgACGGCGCu -3' miRNA: 3'- cuaguaCGGUAuGUGGACG-UGCCGCG- -5' |
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22836 | 5' | -53.8 | NC_005135.1 | + | 72321 | 1.13 | 0.002665 |
Target: 5'- cGAUCAUGCCAUACACCUGCACGGCGCa -3' miRNA: 3'- -CUAGUACGGUAUGUGGACGUGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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