Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22837 | 3' | -45.8 | NC_005135.1 | + | 153877 | 0.66 | 0.999989 |
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Target: 5'- -uUCGCuGAGGCuccaauauaaACUGGGUUAuucgguGUCu -3' miRNA: 3'- guAGCG-CUCCG----------UGACCUAAUuuu---UAG- -5' |
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| 22837 | 3' | -45.8 | NC_005135.1 | + | 88429 | 0.66 | 0.999978 |
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Target: 5'- -uUUGUGaAGGCGC-GGAcggUGAAAAUCa -3' miRNA: 3'- guAGCGC-UCCGUGaCCUa--AUUUUUAG- -5' |
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| 22837 | 3' | -45.8 | NC_005135.1 | + | 109774 | 0.67 | 0.999944 |
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Target: 5'- uGUUGaCGGGGCgaaACUGGAcuUUGAGAcUCg -3' miRNA: 3'- gUAGC-GCUCCG---UGACCU--AAUUUUuAG- -5' |
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| 22837 | 3' | -45.8 | NC_005135.1 | + | 25562 | 0.67 | 0.999926 |
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Target: 5'- uCAUCGCucGGCGacgUUGGA--GAAGAUCg -3' miRNA: 3'- -GUAGCGcuCCGU---GACCUaaUUUUUAG- -5' |
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| 22837 | 3' | -45.8 | NC_005135.1 | + | 14588 | 0.68 | 0.999722 |
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Target: 5'- uUAUCGCGAugGGCA-UGGAUggcaacGAAAUCu -3' miRNA: 3'- -GUAGCGCU--CCGUgACCUAau----UUUUAG- -5' |
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| 22837 | 3' | -45.8 | NC_005135.1 | + | 19498 | 0.68 | 0.999722 |
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Target: 5'- cCAUCGCGAauuuGGUAUUGGAcgUUGuuggugcacauAAAAUCa -3' miRNA: 3'- -GUAGCGCU----CCGUGACCU--AAU-----------UUUUAG- -5' |
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| 22837 | 3' | -45.8 | NC_005135.1 | + | 134165 | 0.68 | 0.999646 |
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Target: 5'- --cCGUagaGGGCAUUcGGAUUAAAAAUCg -3' miRNA: 3'- guaGCGc--UCCGUGA-CCUAAUUUUUAG- -5' |
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| 22837 | 3' | -45.8 | NC_005135.1 | + | 168478 | 0.7 | 0.998932 |
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Target: 5'- cCAggGCGGGGUcuCaGGAUUAGAAGUCu -3' miRNA: 3'- -GUagCGCUCCGu-GaCCUAAUUUUUAG- -5' |
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| 22837 | 3' | -45.8 | NC_005135.1 | + | 74427 | 1.12 | 0.021017 |
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Target: 5'- uCAUCGCGAGGCACUGGAUUAAAAAUCg -3' miRNA: 3'- -GUAGCGCUCCGUGACCUAAUUUUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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