Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22839 | 5' | -44 | NC_005135.1 | + | 159069 | 0.66 | 0.999999 |
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Target: 5'- -uUAcCCAAACCaUGCGAGAUUu---- -3' miRNA: 3'- auAUuGGUUUGG-ACGCUCUAAcauuu -5' |
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| 22839 | 5' | -44 | NC_005135.1 | + | 35197 | 0.67 | 0.999997 |
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Target: 5'- ---cGCCAGACCUGUGcGAcgGUAc- -3' miRNA: 3'- auauUGGUUUGGACGCuCUaaCAUuu -5' |
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| 22839 | 5' | -44 | NC_005135.1 | + | 3000 | 0.67 | 0.999988 |
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Target: 5'- --gAGCCAAACCUGgGAGuccagauaGUUGUu-- -3' miRNA: 3'- auaUUGGUUUGGACgCUC--------UAACAuuu -5' |
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| 22839 | 5' | -44 | NC_005135.1 | + | 45006 | 0.69 | 0.99994 |
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Target: 5'- --cAACCGAACC-GCGAGg--GUGAAg -3' miRNA: 3'- auaUUGGUUUGGaCGCUCuaaCAUUU- -5' |
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| 22839 | 5' | -44 | NC_005135.1 | + | 109562 | 0.72 | 0.996832 |
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Target: 5'- -cUAACCAAACCUGCG-GA--GUAAu -3' miRNA: 3'- auAUUGGUUUGGACGCuCUaaCAUUu -5' |
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| 22839 | 5' | -44 | NC_005135.1 | + | 109404 | 1.05 | 0.063362 |
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Target: 5'- uUAUAACCAAACCUGCGAGAUUGUAAAu -3' miRNA: 3'- -AUAUUGGUUUGGACGCUCUAACAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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