Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22843 | 3' | -42.6 | NC_005135.1 | + | 173171 | 0.66 | 1 |
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Target: 5'- cGAAGCGGAUAAagaacuggaauguacCUGgaUGGCCAa-- -3' miRNA: 3'- cCUUUGCUUAUU---------------GACgaACUGGUaac -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 140474 | 0.68 | 1 |
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Target: 5'- aGGAAGCGcaauaccuUGACUGaUUUGACCGc-- -3' miRNA: 3'- -CCUUUGCuu------AUUGAC-GAACUGGUaac -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 61534 | 0.66 | 1 |
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Target: 5'- uGAAACGAAUAGCU--UUGugCAa-- -3' miRNA: 3'- cCUUUGCUUAUUGAcgAACugGUaac -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 41900 | 0.67 | 1 |
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Target: 5'- -aGAACGAAUAuccugucgaauuggaAUUGUUUGACCAg-- -3' miRNA: 3'- ccUUUGCUUAU---------------UGACGAACUGGUaac -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 17221 | 0.66 | 1 |
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Target: 5'- ---cGCGAAUggUUGCUUGAgCGg-- -3' miRNA: 3'- ccuuUGCUUAuuGACGAACUgGUaac -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 99255 | 0.66 | 1 |
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Target: 5'- uGAGGCGAAgaugaAAUUGCUaccGCCAUUGc -3' miRNA: 3'- cCUUUGCUUa----UUGACGAac-UGGUAAC- -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 157885 | 0.69 | 0.999999 |
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Target: 5'- uGGccAGGCGGAUGGCUGCUUcauGCCGg-- -3' miRNA: 3'- -CC--UUUGCUUAUUGACGAAc--UGGUaac -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 96162 | 0.69 | 0.999999 |
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Target: 5'- --uGACGA---ACUGCUUGACCu--- -3' miRNA: 3'- ccuUUGCUuauUGACGAACUGGuaac -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 169859 | 0.74 | 0.999196 |
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Target: 5'- cGGguACcAAUGGCUGCUgcagauguauUGGCCGUUGa -3' miRNA: 3'- -CCuuUGcUUAUUGACGA----------ACUGGUAAC- -5' |
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| 22843 | 3' | -42.6 | NC_005135.1 | + | 142719 | 1.13 | 0.045051 |
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Target: 5'- aGGAAACGAAUAACUGCUUGACCAUUGc -3' miRNA: 3'- -CCUUUGCUUAUUGACGAACUGGUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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