Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22845 | 3' | -42.3 | NC_005135.1 | + | 46476 | 0.66 | 1 |
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Target: 5'- --aUGAUCggUGUuggcgcugcaaugugUGCUGUAGguGAa -3' miRNA: 3'- uuaAUUAGuuGCA---------------ACGAUAUCguCUg -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 115298 | 0.67 | 1 |
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Target: 5'- --cUGAUCAACGgUGauuucuUGUGGCAGAa -3' miRNA: 3'- uuaAUUAGUUGCaACg-----AUAUCGUCUg -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 28968 | 0.66 | 1 |
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Target: 5'- --gUAAUCAuuGCG-UGCUuUGGCAGGa -3' miRNA: 3'- uuaAUUAGU--UGCaACGAuAUCGUCUg -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 27258 | 0.68 | 1 |
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Target: 5'- ------aCGACGcUGCUGUAGCuGAUc -3' miRNA: 3'- uuaauuaGUUGCaACGAUAUCGuCUG- -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 166108 | 0.67 | 1 |
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Target: 5'- --aUAGUCAugACGUUG-UAUGGCAG-Ca -3' miRNA: 3'- uuaAUUAGU--UGCAACgAUAUCGUCuG- -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 168400 | 0.69 | 0.999996 |
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Target: 5'- uAUUAAUCcACGUUGauaaccAGCAGGCg -3' miRNA: 3'- uUAAUUAGuUGCAACgaua--UCGUCUG- -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 111733 | 0.72 | 0.999821 |
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Target: 5'- ----cAUCAAagacaaagUGCUGUGGCAGACg -3' miRNA: 3'- uuaauUAGUUgca-----ACGAUAUCGUCUG- -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 105010 | 0.73 | 0.999613 |
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Target: 5'- --cUGAUCAACGcUGCUAUcGGCgaAGGCa -3' miRNA: 3'- uuaAUUAGUUGCaACGAUA-UCG--UCUG- -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 97711 | 0.74 | 0.997804 |
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Target: 5'- ---aAAUCAGgugcgacguggUGUUGCUGUGGUAGACc -3' miRNA: 3'- uuaaUUAGUU-----------GCAACGAUAUCGUCUG- -5' |
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| 22845 | 3' | -42.3 | NC_005135.1 | + | 145201 | 1.1 | 0.051821 |
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Target: 5'- aAAUUAAUCAACGUUGCUAUAGCAGACa -3' miRNA: 3'- -UUAAUUAGUUGCAACGAUAUCGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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