Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22846 | 3' | -54.2 | NC_005135.1 | + | 168512 | 0.66 | 0.956856 |
Target: 5'- uGAGCCGGcgAAGCCGGCUCUuCGu--- -3' miRNA: 3'- -UUCGGCUa-UUCGGCUGAGGcGUucuc -5' |
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22846 | 3' | -54.2 | NC_005135.1 | + | 168704 | 0.66 | 0.952902 |
Target: 5'- aGAGCCGGcUucGCCGGCUCaaaaGCAGa-- -3' miRNA: 3'- -UUCGGCU-AuuCGGCUGAGg---CGUUcuc -5' |
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22846 | 3' | -54.2 | NC_005135.1 | + | 156031 | 0.66 | 0.950417 |
Target: 5'- cAAGCCGAgcgcgaucgaaaguuUGAGauGGCUgCGCAAGAa -3' miRNA: 3'- -UUCGGCU---------------AUUCggCUGAgGCGUUCUc -5' |
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22846 | 3' | -54.2 | NC_005135.1 | + | 163766 | 0.68 | 0.906399 |
Target: 5'- aGAGCCcAUAcGCCGACUUCaaGCGGGAu -3' miRNA: 3'- -UUCGGcUAUuCGGCUGAGG--CGUUCUc -5' |
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22846 | 3' | -54.2 | NC_005135.1 | + | 162408 | 0.68 | 0.886503 |
Target: 5'- cAAGCCGAUuGGCuaugauCGACcucCCGCGAGAa -3' miRNA: 3'- -UUCGGCUAuUCG------GCUGa--GGCGUUCUc -5' |
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22846 | 3' | -54.2 | NC_005135.1 | + | 151210 | 1.06 | 0.006388 |
Target: 5'- gAAGCCGAUAAGCCGACUCCGCAAGAGu -3' miRNA: 3'- -UUCGGCUAUUCGGCUGAGGCGUUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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