miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22846 3' -54.2 NC_005135.1 + 168512 0.66 0.956856
Target:  5'- uGAGCCGGcgAAGCCGGCUCUuCGu--- -3'
miRNA:   3'- -UUCGGCUa-UUCGGCUGAGGcGUucuc -5'
22846 3' -54.2 NC_005135.1 + 168704 0.66 0.952902
Target:  5'- aGAGCCGGcUucGCCGGCUCaaaaGCAGa-- -3'
miRNA:   3'- -UUCGGCU-AuuCGGCUGAGg---CGUUcuc -5'
22846 3' -54.2 NC_005135.1 + 156031 0.66 0.950417
Target:  5'- cAAGCCGAgcgcgaucgaaaguuUGAGauGGCUgCGCAAGAa -3'
miRNA:   3'- -UUCGGCU---------------AUUCggCUGAgGCGUUCUc -5'
22846 3' -54.2 NC_005135.1 + 163766 0.68 0.906399
Target:  5'- aGAGCCcAUAcGCCGACUUCaaGCGGGAu -3'
miRNA:   3'- -UUCGGcUAUuCGGCUGAGG--CGUUCUc -5'
22846 3' -54.2 NC_005135.1 + 162408 0.68 0.886503
Target:  5'- cAAGCCGAUuGGCuaugauCGACcucCCGCGAGAa -3'
miRNA:   3'- -UUCGGCUAuUCG------GCUGa--GGCGUUCUc -5'
22846 3' -54.2 NC_005135.1 + 151210 1.06 0.006388
Target:  5'- gAAGCCGAUAAGCCGACUCCGCAAGAGu -3'
miRNA:   3'- -UUCGGCUAUUCGGCUGAGGCGUUCUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.