Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22847 | 5' | -52.8 | NC_005135.1 | + | 108678 | 0.66 | 0.984562 |
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Target: 5'- uGAGAAagGGuuuuGGCUCCGCCUcauguaACAGa- -3' miRNA: 3'- gCUUUUagCC----UCGAGGCGGA------UGUCga -5' |
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| 22847 | 5' | -52.8 | NC_005135.1 | + | 31625 | 0.66 | 0.975879 |
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Target: 5'- aGAAuagCGGcAGUUuuGCCUGCcGCUg -3' miRNA: 3'- gCUUuuaGCC-UCGAggCGGAUGuCGA- -5' |
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| 22847 | 5' | -52.8 | NC_005135.1 | + | 45790 | 0.67 | 0.964069 |
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Target: 5'- uCGGAugcUCGGAGCaUCCGauaaCUACAuGCg -3' miRNA: 3'- -GCUUuu-AGCCUCG-AGGCg---GAUGU-CGa -5' |
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| 22847 | 5' | -52.8 | NC_005135.1 | + | 64983 | 0.71 | 0.823648 |
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Target: 5'- gGAAGAUUGGAGaaCCGCCgauaaAGCUg -3' miRNA: 3'- gCUUUUAGCCUCgaGGCGGaug--UCGA- -5' |
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| 22847 | 5' | -52.8 | NC_005135.1 | + | 78768 | 0.74 | 0.709388 |
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Target: 5'- aGAGAAUCGaGAGCUUCaUCUGCGGCc -3' miRNA: 3'- gCUUUUAGC-CUCGAGGcGGAUGUCGa -5' |
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| 22847 | 5' | -52.8 | NC_005135.1 | + | 152840 | 1.09 | 0.006759 |
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Target: 5'- uCGAAAAUCGGAGCUCCGCCUACAGCUa -3' miRNA: 3'- -GCUUUUAGCCUCGAGGCGGAUGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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