Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22849 | 3' | -56.5 | NC_005135.1 | + | 173400 | 0.66 | 0.904457 |
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Target: 5'- cUGCAAGCCAGGUAGUUcCGGa---- -3' miRNA: 3'- aGCGUUCGGUCCGUCGGuGCUgaucg -5' |
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| 22849 | 3' | -56.5 | NC_005135.1 | + | 72497 | 0.66 | 0.904457 |
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Target: 5'- aUCGCAGGCgCuuGGCuGCC---ACUAGCc -3' miRNA: 3'- -AGCGUUCG-Gu-CCGuCGGugcUGAUCG- -5' |
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| 22849 | 3' | -56.5 | NC_005135.1 | + | 55991 | 0.66 | 0.898122 |
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Target: 5'- gCGCAGGCUGuccguaucaauGGCGGUaccgaGCGACaAGCu -3' miRNA: 3'- aGCGUUCGGU-----------CCGUCGg----UGCUGaUCG- -5' |
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| 22849 | 3' | -56.5 | NC_005135.1 | + | 145170 | 0.66 | 0.884764 |
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Target: 5'- -aGCGA-CCA-GCGGCUuagauGCGGCUGGCg -3' miRNA: 3'- agCGUUcGGUcCGUCGG-----UGCUGAUCG- -5' |
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| 22849 | 3' | -56.5 | NC_005135.1 | + | 35008 | 0.66 | 0.884764 |
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Target: 5'- aUCGauuuuGGUCAGaGCAGCCAgcuCGACUacaucaucAGCg -3' miRNA: 3'- -AGCgu---UCGGUC-CGUCGGU---GCUGA--------UCG- -5' |
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| 22849 | 3' | -56.5 | NC_005135.1 | + | 55442 | 0.67 | 0.855425 |
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Target: 5'- uUCGCGAGCUucucGuGCGGCCuCGGC-AGUc -3' miRNA: 3'- -AGCGUUCGGu---C-CGUCGGuGCUGaUCG- -5' |
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| 22849 | 3' | -56.5 | NC_005135.1 | + | 26610 | 0.67 | 0.847574 |
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Target: 5'- cUUGCAGGCgCucgauGGCAGCUuuuGCGGCUuucGCc -3' miRNA: 3'- -AGCGUUCG-Gu----CCGUCGG---UGCUGAu--CG- -5' |
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| 22849 | 3' | -56.5 | NC_005135.1 | + | 90967 | 0.67 | 0.831296 |
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Target: 5'- aCGCuucGuCCAGGUGGUCAuaugUGGCUGGCu -3' miRNA: 3'- aGCGuu-C-GGUCCGUCGGU----GCUGAUCG- -5' |
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| 22849 | 3' | -56.5 | NC_005135.1 | + | 163961 | 1.13 | 0.001471 |
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Target: 5'- aUCGCAAGCCAGGCAGCCACGACUAGCg -3' miRNA: 3'- -AGCGUUCGGUCCGUCGGUGCUGAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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