Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22849 | 5' | -48.2 | NC_005135.1 | + | 10975 | 0.66 | 0.999642 |
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Target: 5'- gGGUCAGUUCGccuaucauGGCACCaacuGAUUuaGCGa -3' miRNA: 3'- gCCAGUUAAGUuu------CCGUGG----CUAA--CGU- -5' |
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| 22849 | 5' | -48.2 | NC_005135.1 | + | 132288 | 0.68 | 0.99775 |
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Target: 5'- uGGUUucaaUCAAcGGCACCGGUaucgGCAc -3' miRNA: 3'- gCCAGuua-AGUUuCCGUGGCUAa---CGU- -5' |
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| 22849 | 5' | -48.2 | NC_005135.1 | + | 170351 | 0.68 | 0.99775 |
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Target: 5'- gGGUCGAagUCuguuGGCACCGGaugGCGc -3' miRNA: 3'- gCCAGUUa-AGuuu-CCGUGGCUaa-CGU- -5' |
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| 22849 | 5' | -48.2 | NC_005135.1 | + | 71743 | 0.68 | 0.996818 |
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Target: 5'- aCGGgcgCAGcgagUCGAuuGGCAUUGAUUGCGa -3' miRNA: 3'- -GCCa--GUUa---AGUUu-CCGUGGCUAACGU- -5' |
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| 22849 | 5' | -48.2 | NC_005135.1 | + | 32082 | 0.69 | 0.995587 |
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Target: 5'- gGGUCGc-UCAgucGAGGUGCCGAgcaaUUGCAu -3' miRNA: 3'- gCCAGUuaAGU---UUCCGUGGCU----AACGU- -5' |
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| 22849 | 5' | -48.2 | NC_005135.1 | + | 163997 | 1.1 | 0.014569 |
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Target: 5'- cCGGUCAAUUCAAAGGCACCGAUUGCAa -3' miRNA: 3'- -GCCAGUUAAGUUUCCGUGGCUAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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