Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22857 | 3' | -51.8 | NC_005137.2 | + | 123656 | 1.12 | 0.004051 |
Target: 5'- gCCGGCACCAGCAACAUGACUCAACUGg -3' miRNA: 3'- -GGCCGUGGUCGUUGUACUGAGUUGAC- -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 22394 | 0.75 | 0.652065 |
Target: 5'- cCCuacGCACCGGCAACAUGGCgCGcCUGa -3' miRNA: 3'- -GGc--CGUGGUCGUUGUACUGaGUuGAC- -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 95445 | 0.73 | 0.735795 |
Target: 5'- aUGGCGCCGGCGguGCGUGuCUUGACa- -3' miRNA: 3'- gGCCGUGGUCGU--UGUACuGAGUUGac -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 32655 | 0.72 | 0.798529 |
Target: 5'- aCCGGCGCacaAGCGugacggugacguacgACAUGACgUCAACa- -3' miRNA: 3'- -GGCCGUGg--UCGU---------------UGUACUG-AGUUGac -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 64530 | 0.72 | 0.813273 |
Target: 5'- gUGcGCACCGGCAACGUuGAC-CAACg- -3' miRNA: 3'- gGC-CGUGGUCGUUGUA-CUGaGUUGac -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 87939 | 0.71 | 0.848055 |
Target: 5'- aCCGGUugC-GCGucACAaauUGACUUAACUGg -3' miRNA: 3'- -GGCCGugGuCGU--UGU---ACUGAGUUGAC- -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 28897 | 0.7 | 0.864205 |
Target: 5'- gUGGCGUCGGUGAUAUGGCUUAugUGc -3' miRNA: 3'- gGCCGUGGUCGUUGUACUGAGUugAC- -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 101428 | 0.7 | 0.879449 |
Target: 5'- uUGGCACCAccucCAACGgcUGAUUCAAUUGg -3' miRNA: 3'- gGCCGUGGUc---GUUGU--ACUGAGUUGAC- -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 113880 | 0.7 | 0.879449 |
Target: 5'- gCUGGUGaCGGCAAUGUGGCUC-GCUGa -3' miRNA: 3'- -GGCCGUgGUCGUUGUACUGAGuUGAC- -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 25487 | 0.69 | 0.907037 |
Target: 5'- aCGGUGCCAGCAGCcguGUGGCgCcGCUu -3' miRNA: 3'- gGCCGUGGUCGUUG---UACUGaGuUGAc -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 7364 | 0.69 | 0.925071 |
Target: 5'- gCUGGUGCCGGCAaaauaGCGUGGgucUUCAGCg- -3' miRNA: 3'- -GGCCGUGGUCGU-----UGUACU---GAGUUGac -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 15751 | 0.68 | 0.9358 |
Target: 5'- gCGGCGCCAGCGucgcCAUGugUucguCGACc- -3' miRNA: 3'- gGCCGUGGUCGUu---GUACugA----GUUGac -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 31228 | 0.68 | 0.93931 |
Target: 5'- gCGGCacACCAGCuugAACAUGgccgcgucgugcgcGCUCAACUc -3' miRNA: 3'- gGCCG--UGGUCG---UUGUAC--------------UGAGUUGAc -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 5958 | 0.68 | 0.940777 |
Target: 5'- uCCGGCGCCGcGCcGCccGGCUCcgUUGc -3' miRNA: 3'- -GGCCGUGGU-CGuUGuaCUGAGuuGAC- -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 32335 | 0.68 | 0.940777 |
Target: 5'- gUGGC-CCAGCAGCAcGA-UCAGCUc -3' miRNA: 3'- gGCCGuGGUCGUUGUaCUgAGUUGAc -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 20575 | 0.68 | 0.949964 |
Target: 5'- aCCGaGgACCucGCGACAUGACcgUCGACg- -3' miRNA: 3'- -GGC-CgUGGu-CGUUGUACUG--AGUUGac -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 121542 | 0.67 | 0.954179 |
Target: 5'- aCCGGCGCgaaAGCAccACGUGgcacaauaauuuGCUCAaaauGCUGg -3' miRNA: 3'- -GGCCGUGg--UCGU--UGUAC------------UGAGU----UGAC- -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 59315 | 0.67 | 0.958148 |
Target: 5'- gCGGCACCAGCAccucGAUcCGGCUa -3' miRNA: 3'- gGCCGUGGUCGUuguaCUGaGUUGAc -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 125017 | 0.67 | 0.958148 |
Target: 5'- aCCGGCgAUCGguuGCGGCGUGGCgcaggCAACg- -3' miRNA: 3'- -GGCCG-UGGU---CGUUGUACUGa----GUUGac -5' |
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22857 | 3' | -51.8 | NC_005137.2 | + | 124964 | 0.67 | 0.958148 |
Target: 5'- gCGGCGCCGGUGAUggGGCgcugcGCUGc -3' miRNA: 3'- gGCCGUGGUCGUUGuaCUGagu--UGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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