Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22857 | 3' | -51.8 | NC_005137.2 | + | 7364 | 0.69 | 0.925071 |
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Target: 5'- gCUGGUGCCGGCAaaauaGCGUGGgucUUCAGCg- -3' miRNA: 3'- -GGCCGUGGUCGU-----UGUACU---GAGUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 113880 | 0.7 | 0.879449 |
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Target: 5'- gCUGGUGaCGGCAAUGUGGCUC-GCUGa -3' miRNA: 3'- -GGCCGUgGUCGUUGUACUGAGuUGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 101428 | 0.7 | 0.879449 |
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Target: 5'- uUGGCACCAccucCAACGgcUGAUUCAAUUGg -3' miRNA: 3'- gGCCGUGGUc---GUUGU--ACUGAGUUGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 28897 | 0.7 | 0.864205 |
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Target: 5'- gUGGCGUCGGUGAUAUGGCUUAugUGc -3' miRNA: 3'- gGCCGUGGUCGUUGUACUGAGUugAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 87939 | 0.71 | 0.848055 |
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Target: 5'- aCCGGUugC-GCGucACAaauUGACUUAACUGg -3' miRNA: 3'- -GGCCGugGuCGU--UGU---ACUGAGUUGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 32655 | 0.72 | 0.798529 |
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Target: 5'- aCCGGCGCacaAGCGugacggugacguacgACAUGACgUCAACa- -3' miRNA: 3'- -GGCCGUGg--UCGU---------------UGUACUG-AGUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 64530 | 0.72 | 0.813273 |
|
Target: 5'- gUGcGCACCGGCAACGUuGAC-CAACg- -3' miRNA: 3'- gGC-CGUGGUCGUUGUA-CUGaGUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 95445 | 0.73 | 0.735795 |
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Target: 5'- aUGGCGCCGGCGguGCGUGuCUUGACa- -3' miRNA: 3'- gGCCGUGGUCGU--UGUACuGAGUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 22394 | 0.75 | 0.652065 |
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Target: 5'- cCCuacGCACCGGCAACAUGGCgCGcCUGa -3' miRNA: 3'- -GGc--CGUGGUCGUUGUACUGaGUuGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 123656 | 1.12 | 0.004051 |
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Target: 5'- gCCGGCACCAGCAACAUGACUCAACUGg -3' miRNA: 3'- -GGCCGUGGUCGUUGUACUGAGUUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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