miRNA: 3'- -UCGUGaaACUGgCGU-AGGACGGGCCc -5'
miRNA: 3'- -UCGUgaaaCugGCGuaGGACGGGCCC- -5'
miRNA: 3'- -UCGUGaaACUGGCGUAGGaCGGGCCc -5'
miRNA: 3'- -UCGUGAAACUGGCGUAGGACGGGCCC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22857 | 5' | -56.7 | NC_005137.2 | + | 8050 | 0.67 | 0.790658 |
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Target: 5'- cGGUGC--UGACgGCGuUCCUGCUCGGc -3' miRNA: 3'- -UCGUGaaACUGgCGU-AGGACGGGCCc -5' |
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| 22857 | 5' | -56.7 | NC_005137.2 | + | 78733 | 0.71 | 0.570572 |
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Target: 5'- cGGCGauaaGugCGCggUCUGCCCGGGc -3' miRNA: 3'- -UCGUgaaaCugGCGuaGGACGGGCCC- -5' |
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| 22857 | 5' | -56.7 | NC_005137.2 | + | 71593 | 0.72 | 0.530388 |
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Target: 5'- gGGCGCgcUGGCCGUGUUCaGCCUGGu -3' miRNA: 3'- -UCGUGaaACUGGCGUAGGaCGGGCCc -5' |
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| 22857 | 5' | -56.7 | NC_005137.2 | + | 123692 | 1.09 | 0.001956 |
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Target: 5'- cAGCACUUUGACCGCAUCCUGCCCGGGc -3' miRNA: 3'- -UCGUGAAACUGGCGUAGGACGGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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