Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22858 | 3' | -57.6 | NC_005137.2 | + | 80356 | 0.66 | 0.761922 |
Target: 5'- aGCGGUcaaauaugaGGGCGGCaag-GUGCUGCAGc -3' miRNA: 3'- aCGCCA---------CCCGCCGauaaCGUGGUGUU- -5' |
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22858 | 3' | -57.6 | NC_005137.2 | + | 70594 | 0.66 | 0.752151 |
Target: 5'- cUGCGc-GGGCGGCUuuUUGUugCGCu- -3' miRNA: 3'- -ACGCcaCCCGCCGAu-AACGugGUGuu -5' |
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22858 | 3' | -57.6 | NC_005137.2 | + | 103811 | 0.66 | 0.742271 |
Target: 5'- uUGCuGGUGugcGGCGGCggguuggUGCACgGCAu -3' miRNA: 3'- -ACG-CCAC---CCGCCGaua----ACGUGgUGUu -5' |
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22858 | 3' | -57.6 | NC_005137.2 | + | 53095 | 0.67 | 0.712066 |
Target: 5'- gUGCaGGUGGGUGuGUUugcgGUUGCACgACGc -3' miRNA: 3'- -ACG-CCACCCGC-CGA----UAACGUGgUGUu -5' |
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22858 | 3' | -57.6 | NC_005137.2 | + | 75584 | 0.67 | 0.681222 |
Target: 5'- gGCGGacgcGGGCGGCgccgUGCGCguggaGCAAg -3' miRNA: 3'- aCGCCa---CCCGCCGaua-ACGUGg----UGUU- -5' |
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22858 | 3' | -57.6 | NC_005137.2 | + | 8635 | 0.68 | 0.670843 |
Target: 5'- gGCGGgcgUGGGCGuGCUAUUG-GCCGgAGg -3' miRNA: 3'- aCGCC---ACCCGC-CGAUAACgUGGUgUU- -5' |
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22858 | 3' | -57.6 | NC_005137.2 | + | 103580 | 0.68 | 0.618637 |
Target: 5'- aGCGcGUGGGCcaGCUGUUacaGCGCUGCAGc -3' miRNA: 3'- aCGC-CACCCGc-CGAUAA---CGUGGUGUU- -5' |
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22858 | 3' | -57.6 | NC_005137.2 | + | 123537 | 1.06 | 0.002018 |
Target: 5'- uUGCGGUGGGCGGCUAUUGCACCACAAa -3' miRNA: 3'- -ACGCCACCCGCCGAUAACGUGGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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