Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22859 | 3' | -53.9 | NC_005137.2 | + | 78747 | 0.66 | 0.933419 |
Target: 5'- -cGGUcUGCCcgGGCGccguuuguaaccagAGCUucuGCGGCGa -3' miRNA: 3'- uuCCAaACGGuaCCGU--------------UCGG---UGCUGC- -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 90529 | 0.66 | 0.929695 |
Target: 5'- -uGGUUaGCguUGGCGAGCguCACGAUc -3' miRNA: 3'- uuCCAAaCGguACCGUUCG--GUGCUGc -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 17237 | 0.66 | 0.929695 |
Target: 5'- gGAGGcg-GUCAUGGCG-GCUaaACGACa -3' miRNA: 3'- -UUCCaaaCGGUACCGUuCGG--UGCUGc -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 11282 | 0.66 | 0.929695 |
Target: 5'- cAGG-UUGCCAUGGgc-GCUgcGCGGCGg -3' miRNA: 3'- uUCCaAACGGUACCguuCGG--UGCUGC- -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 97364 | 0.66 | 0.914752 |
Target: 5'- -cGGUUUGCCG-GGCAGaaacuugaacacguuGCCGCG-Ca -3' miRNA: 3'- uuCCAAACGGUaCCGUU---------------CGGUGCuGc -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 78368 | 0.66 | 0.905983 |
Target: 5'- gGAGGUUgcUGCgGUGGU-GGCUgagGCGGCGg -3' miRNA: 3'- -UUCCAA--ACGgUACCGuUCGG---UGCUGC- -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 45475 | 0.66 | 0.905337 |
Target: 5'- --cGUUUGUCAUGGUuucguuucuggacAAGUCGCGACc -3' miRNA: 3'- uucCAAACGGUACCG-------------UUCGGUGCUGc -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 99745 | 0.68 | 0.852422 |
Target: 5'- -cGGUa-GCCAUGGCGagcacuaauuguuuGGCCGCG-CGa -3' miRNA: 3'- uuCCAaaCGGUACCGU--------------UCGGUGCuGC- -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 9245 | 0.7 | 0.764123 |
Target: 5'- -uGGcUUGCCAUGGCAAaCCuugaGACGg -3' miRNA: 3'- uuCCaAACGGUACCGUUcGGug--CUGC- -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 86088 | 0.71 | 0.681936 |
Target: 5'- -cGGc--GUCAUGGUAcGCCGCGACGa -3' miRNA: 3'- uuCCaaaCGGUACCGUuCGGUGCUGC- -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 30015 | 0.75 | 0.454156 |
Target: 5'- -cGGUUggacaGgCGUGGCAGGCUAUGACGg -3' miRNA: 3'- uuCCAAa----CgGUACCGUUCGGUGCUGC- -5' |
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22859 | 3' | -53.9 | NC_005137.2 | + | 121764 | 1.06 | 0.004773 |
Target: 5'- cAAGGUUUGCCAUGGCAAGCCACGACGu -3' miRNA: 3'- -UUCCAAACGGUACCGUUCGGUGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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