Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22859 | 5' | -49.9 | NC_005137.2 | + | 121804 | 1.11 | 0.00698 |
Target: 5'- uGCCAUCGCGUUUGCCAUCACAACUGUa -3' miRNA: 3'- -CGGUAGCGCAAACGGUAGUGUUGACA- -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 16776 | 0.7 | 0.926624 |
Target: 5'- cGgCAcCGCGUUUGCCAaCAC-GCUGg -3' miRNA: 3'- -CgGUaGCGCAAACGGUaGUGuUGACa -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 47562 | 0.7 | 0.926624 |
Target: 5'- gGCUggCGCGUacUUGCCAgugCACAGCa-- -3' miRNA: 3'- -CGGuaGCGCA--AACGGUa--GUGUUGaca -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 2245 | 0.69 | 0.951525 |
Target: 5'- cGCgAUCGCGUUuaccuuUGCgAUUACgGGCUGUg -3' miRNA: 3'- -CGgUAGCGCAA------ACGgUAGUG-UUGACA- -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 20376 | 0.69 | 0.962993 |
Target: 5'- cGCCGUcggCGCGUUUgugcacgGCCGUCACcacggacaccAACUGg -3' miRNA: 3'- -CGGUA---GCGCAAA-------CGGUAGUG----------UUGACa -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 93478 | 0.68 | 0.970014 |
Target: 5'- cGCCAgCGcCGUUUGCCGaaACAGCa-- -3' miRNA: 3'- -CGGUaGC-GCAAACGGUagUGUUGaca -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 87835 | 0.68 | 0.978314 |
Target: 5'- cGCUugCGCGUUUGUCAUUAUGACg-- -3' miRNA: 3'- -CGGuaGCGCAAACGGUAGUGUUGaca -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 55724 | 0.68 | 0.980661 |
Target: 5'- cGCCGUUGCcc--GCCGUUACAGCcGg -3' miRNA: 3'- -CGGUAGCGcaaaCGGUAGUGUUGaCa -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 3939 | 0.67 | 0.988193 |
Target: 5'- uCCAUCGCGUUuauuagUGCCAU-GCAugUa- -3' miRNA: 3'- cGGUAGCGCAA------ACGGUAgUGUugAca -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 44327 | 0.66 | 0.990981 |
Target: 5'- gGCCAUgGCGacgUGCguUCAaguCGGCUGUu -3' miRNA: 3'- -CGGUAgCGCaa-ACGguAGU---GUUGACA- -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 41827 | 0.66 | 0.991471 |
Target: 5'- cGCCA-CGCGUUUGUUAgcgcuggugaggugCACGAUUGc -3' miRNA: 3'- -CGGUaGCGCAAACGGUa-------------GUGUUGACa -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 20588 | 0.66 | 0.991708 |
Target: 5'- aCCGUCGCGcccgUUGCCAgcugcggaaacacCACGuCUGUa -3' miRNA: 3'- cGGUAGCGCa---AACGGUa------------GUGUuGACA- -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 48058 | 0.66 | 0.994163 |
Target: 5'- uGCCG-CGCGU--GCCAUCuCGACg-- -3' miRNA: 3'- -CGGUaGCGCAaaCGGUAGuGUUGaca -5' |
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22859 | 5' | -49.9 | NC_005137.2 | + | 11062 | 0.66 | 0.994995 |
Target: 5'- uGCUugCGCGUguggcUGCCAacUUGCAACUGa -3' miRNA: 3'- -CGGuaGCGCAa----ACGGU--AGUGUUGACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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