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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22860 | 3' | -52.6 | NC_005137.2 | + | 5989 | 0.69 | 0.842148 |
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Target: 5'- cGCCACGCCgcaaccgaucGCCGGUGcGGC-AUCc -3' miRNA: 3'- uCGGUGCGGa---------CGGCUAUuUCGaUAGu -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 25743 | 0.66 | 0.963444 |
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Target: 5'- cGGCCaggACGUUUGCCGAUAAA-CUAa-- -3' miRNA: 3'- -UCGG---UGCGGACGGCUAUUUcGAUagu -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 39586 | 0.66 | 0.951761 |
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Target: 5'- cGCgACGCCUGCgCGuuUAAGGUauugGUCGg -3' miRNA: 3'- uCGgUGCGGACG-GCu-AUUUCGa---UAGU- -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 42997 | 0.68 | 0.90996 |
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Target: 5'- uGGCCGCGCCgGCaaucccauuaucGAAGCUAUCc -3' miRNA: 3'- -UCGGUGCGGaCGgcua--------UUUCGAUAGu -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 47613 | 0.69 | 0.873636 |
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Target: 5'- aAGCCACGCCUGCaauucuaCGGcgUAGGGCa---- -3' miRNA: 3'- -UCGGUGCGGACG-------GCU--AUUUCGauagu -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 80728 | 0.66 | 0.947367 |
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Target: 5'- gGGCCGCGaaaaaCUGaCCGc---GGCUAUCGg -3' miRNA: 3'- -UCGGUGCg----GAC-GGCuauuUCGAUAGU- -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 86000 | 0.71 | 0.74815 |
|
Target: 5'- uGGCCGCGCCgGCCGAgcauaaauUGuuGCUAa-- -3' miRNA: 3'- -UCGGUGCGGaCGGCU--------AUuuCGAUagu -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 98357 | 0.66 | 0.947367 |
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Target: 5'- cGUCACGCUUgugcGCCGGUuuGGCgccGUCGc -3' miRNA: 3'- uCGGUGCGGA----CGGCUAuuUCGa--UAGU- -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 119015 | 1.09 | 0.00414 |
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Target: 5'- aAGCCACGCCUGCCGAUAAAGCUAUCAa -3' miRNA: 3'- -UCGGUGCGGACGGCUAUUUCGAUAGU- -5' |
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| 22860 | 3' | -52.6 | NC_005137.2 | + | 130052 | 0.78 | 0.400307 |
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Target: 5'- aAGCCGCGCCUuauugcGCCGAUGAcgGGCgcugGUCu -3' miRNA: 3'- -UCGGUGCGGA------CGGCUAUU--UCGa---UAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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