Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22861 | 3' | -53.3 | NC_005137.2 | + | 96923 | 0.71 | 0.68666 |
Target: 5'- uAUUUGuuucGCGCCCGCGUGUGCAu-- -3' miRNA: 3'- -UAAACugu-UGCGGGUGCACGCGUcaa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 110654 | 0.7 | 0.738031 |
Target: 5'- cGUUUGGCuugUGCCCgugacggGCGUGCGCGGa- -3' miRNA: 3'- -UAAACUGuu-GCGGG-------UGCACGCGUCaa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 35135 | 0.7 | 0.749271 |
Target: 5'- gGUUUGACAcGCGCCauugaaaACGUGgGCAGc- -3' miRNA: 3'- -UAAACUGU-UGCGGg------UGCACgCGUCaa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 64538 | 0.69 | 0.834794 |
Target: 5'- ---cGGCAACGUugaCCaACGUGCGCAcGUUa -3' miRNA: 3'- uaaaCUGUUGCG---GG-UGCACGCGU-CAA- -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 17401 | 0.68 | 0.859971 |
Target: 5'- cGUUUGcgcGCAAUGCCacuguuucuUACGUGUGCGGUg -3' miRNA: 3'- -UAAAC---UGUUGCGG---------GUGCACGCGUCAa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 116843 | 0.66 | 0.922599 |
Target: 5'- uGUUUGAgGACGCgUACGUGgauuggaGCGGUg -3' miRNA: 3'- -UAAACUgUUGCGgGUGCACg------CGUCAa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 125146 | 0.66 | 0.922599 |
Target: 5'- -cUUGACAAUGCaccgccgcugaCACGaugGCGCGGUg -3' miRNA: 3'- uaAACUGUUGCGg----------GUGCa--CGCGUCAa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 26670 | 0.66 | 0.938807 |
Target: 5'- ---cGACGACGCaCgCACGUGCGaacGUUu -3' miRNA: 3'- uaaaCUGUUGCG-G-GUGCACGCgu-CAA- -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 99956 | 0.66 | 0.943682 |
Target: 5'- ---gGACAACGgCCGCGgccGCGCGc-- -3' miRNA: 3'- uaaaCUGUUGCgGGUGCa--CGCGUcaa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 101025 | 0.75 | 0.456266 |
Target: 5'- --aUGGCAACGCCCACaauaauuuUGCGCGGg- -3' miRNA: 3'- uaaACUGUUGCGGGUGc-------ACGCGUCaa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 118032 | 1.03 | 0.008435 |
Target: 5'- aAUUUGACAACGCCCACGUGCGCAGUUu -3' miRNA: 3'- -UAAACUGUUGCGGGUGCACGCGUCAA- -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 103479 | 0.75 | 0.475887 |
Target: 5'- -gUUGAUAACGgUCAUGUGCGCGGa- -3' miRNA: 3'- uaAACUGUUGCgGGUGCACGCGUCaa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 100003 | 0.73 | 0.579311 |
Target: 5'- ----aGCGGCGCCgaccaGCGUGCGCGGUUg -3' miRNA: 3'- uaaacUGUUGCGGg----UGCACGCGUCAA- -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 16204 | 0.69 | 0.807781 |
Target: 5'- --cUGACGACGaCgACGUGCGCAa-- -3' miRNA: 3'- uaaACUGUUGCgGgUGCACGCGUcaa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 24804 | 0.68 | 0.875629 |
Target: 5'- -cUUGGCGaccGCGuCCCACGUGCGgauGGUg -3' miRNA: 3'- uaAACUGU---UGC-GGGUGCACGCg--UCAa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 55019 | 0.67 | 0.897296 |
Target: 5'- --gUGGCAAUGgCC-CGUGCGCcGUg -3' miRNA: 3'- uaaACUGUUGCgGGuGCACGCGuCAa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 122914 | 0.67 | 0.897296 |
Target: 5'- aAUUUGuAUAugGCCCAUGUG-GUGGUg -3' miRNA: 3'- -UAAAC-UGUugCGGGUGCACgCGUCAa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 80902 | 0.66 | 0.916666 |
Target: 5'- --aUGGCcACGCUCACGcgcauuUGCGCGGc- -3' miRNA: 3'- uaaACUGuUGCGGGUGC------ACGCGUCaa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 7678 | 0.66 | 0.928266 |
Target: 5'- -gUUGGCGcaGCGUCCAUGUGCcuuccaaaGUAGUa -3' miRNA: 3'- uaAACUGU--UGCGGGUGCACG--------CGUCAa -5' |
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22861 | 3' | -53.3 | NC_005137.2 | + | 103082 | 0.66 | 0.933669 |
Target: 5'- uUUUG-CGAUuaCCGCGUGCGCAa-- -3' miRNA: 3'- uAAACuGUUGcgGGUGCACGCGUcaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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