Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22862 | 5' | -52.7 | NC_005137.2 | + | 125759 | 0.66 | 0.974456 |
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Target: 5'- gCCgcGCGuuucgaGCGGCgCGUGGCAAuuuauUUCg -3' miRNA: 3'- gGGa-CGCua----UGCCG-GCACCGUUu----AAG- -5' |
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| 22862 | 5' | -52.7 | NC_005137.2 | + | 84732 | 0.66 | 0.961983 |
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Target: 5'- aUgaGCGAgUugGGuuGUGGCAAAa-- -3' miRNA: 3'- gGgaCGCU-AugCCggCACCGUUUaag -5' |
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| 22862 | 5' | -52.7 | NC_005137.2 | + | 51732 | 0.67 | 0.95191 |
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Target: 5'- gCCCUGUGAUGCGuuacgccaagauuguGCUGUugcaaGGCGAAa-- -3' miRNA: 3'- -GGGACGCUAUGC---------------CGGCA-----CCGUUUaag -5' |
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| 22862 | 5' | -52.7 | NC_005137.2 | + | 19015 | 0.67 | 0.950216 |
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Target: 5'- gCUggGCGAaacguacaaaGCGGCCGUGGUgcAUUCa -3' miRNA: 3'- gGGa-CGCUa---------UGCCGGCACCGuuUAAG- -5' |
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| 22862 | 5' | -52.7 | NC_005137.2 | + | 24308 | 0.68 | 0.925662 |
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Target: 5'- gUUUGCGGcUGCGGUgGUGGCGGGc-- -3' miRNA: 3'- gGGACGCU-AUGCCGgCACCGUUUaag -5' |
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| 22862 | 5' | -52.7 | NC_005137.2 | + | 61360 | 0.68 | 0.919991 |
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Target: 5'- gCUCUGCGucaaauggGCGGCC--GGCGAGUUg -3' miRNA: 3'- -GGGACGCua------UGCCGGcaCCGUUUAAg -5' |
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| 22862 | 5' | -52.7 | NC_005137.2 | + | 96175 | 0.71 | 0.787909 |
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Target: 5'- uCCCauCGAggugACGGCCGUgGGCAAGUa- -3' miRNA: 3'- -GGGacGCUa---UGCCGGCA-CCGUUUAag -5' |
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| 22862 | 5' | -52.7 | NC_005137.2 | + | 57216 | 0.73 | 0.677299 |
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Target: 5'- aCCUGCagca-GGCCGUGGCGAAc-- -3' miRNA: 3'- gGGACGcuaugCCGGCACCGUUUaag -5' |
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| 22862 | 5' | -52.7 | NC_005137.2 | + | 113554 | 1.11 | 0.003533 |
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Target: 5'- aCCCUGCGAUACGGCCGUGGCAAAUUCc -3' miRNA: 3'- -GGGACGCUAUGCCGGCACCGUUUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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