Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22863 | 5' | -50.5 | NC_005137.2 | + | 37440 | 0.66 | 0.984009 |
Target: 5'- -cGUGAuugccGCcgCGCACCACGuauuugcgcucgauCCGGUCa -3' miRNA: 3'- aaCACUu----UGa-GCGUGGUGUu-------------GGUCAG- -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 110088 | 0.67 | 0.978741 |
Target: 5'- uUUG-GAAACcgCGCGCCAgAugCGGUa -3' miRNA: 3'- -AACaCUUUGa-GCGUGGUgUugGUCAg -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 17620 | 0.67 | 0.976202 |
Target: 5'- aUGUGGAcGCuUCGUugCugAGCCAGa- -3' miRNA: 3'- aACACUU-UG-AGCGugGugUUGGUCag -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 50216 | 0.67 | 0.967263 |
Target: 5'- -cGUGGucaagccaAACUCGCGCaGCAGCUcggGGUCg -3' miRNA: 3'- aaCACU--------UUGAGCGUGgUGUUGG---UCAG- -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 8506 | 0.67 | 0.967263 |
Target: 5'- -cGUGAAAaagaCGCGCgGCGACgCAGUUa -3' miRNA: 3'- aaCACUUUga--GCGUGgUGUUG-GUCAG- -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 7261 | 0.68 | 0.960121 |
Target: 5'- -cGUuGAcCUCGCgACCuaACAACCGGUCa -3' miRNA: 3'- aaCAcUUuGAGCG-UGG--UGUUGGUCAG- -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 81952 | 0.68 | 0.947505 |
Target: 5'- -aGUGc-GCUCGCACgGCuACCuGUCg -3' miRNA: 3'- aaCACuuUGAGCGUGgUGuUGGuCAG- -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 87421 | 0.69 | 0.942773 |
Target: 5'- gUGUGuGACUUGgGCaACGACCAGUa -3' miRNA: 3'- aACACuUUGAGCgUGgUGUUGGUCAg -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 110743 | 0.7 | 0.908684 |
Target: 5'- ------cACUCGCGCUugGGCCAGUUu -3' miRNA: 3'- aacacuuUGAGCGUGGugUUGGUCAG- -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 66422 | 0.7 | 0.887998 |
Target: 5'- gUGUcggGAAACUUGCGCaAUAACCAGUa -3' miRNA: 3'- aACA---CUUUGAGCGUGgUGUUGGUCAg -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 110032 | 0.7 | 0.887998 |
Target: 5'- -cGUGcAAGCgcggcaaaacgCGCGCCGCAggcagcagacGCCAGUCg -3' miRNA: 3'- aaCAC-UUUGa----------GCGUGGUGU----------UGGUCAG- -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 119069 | 0.75 | 0.657912 |
Target: 5'- -cGUGGA-CUCGCACCACAGCaaaaucuGUCc -3' miRNA: 3'- aaCACUUuGAGCGUGGUGUUGgu-----CAG- -5' |
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22863 | 5' | -50.5 | NC_005137.2 | + | 110467 | 1.09 | 0.007035 |
Target: 5'- uUUGUGAAACUCGCACCACAACCAGUCg -3' miRNA: 3'- -AACACUUUGAGCGUGGUGUUGGUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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