Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22865 | 3' | -57.1 | NC_005137.2 | + | 112467 | 0.69 | 0.660951 |
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Target: 5'- gGAUGGGC--GACGGCGCCGUGUa--- -3' miRNA: 3'- -UUGCUCGcaCUGCCGCGGUACAgcuc -5' |
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| 22865 | 3' | -57.1 | NC_005137.2 | + | 105500 | 1.05 | 0.003295 |
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Target: 5'- cAACGAGCGUGACGGCGCCAUGUCGAGc -3' miRNA: 3'- -UUGCUCGCACUGCCGCGGUACAGCUC- -5' |
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| 22865 | 3' | -57.1 | NC_005137.2 | + | 99731 | 0.66 | 0.807128 |
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Target: 5'- uGCGuaGGUGuUGACGGUaGCCAUGgCGAGc -3' miRNA: 3'- uUGC--UCGC-ACUGCCG-CGGUACaGCUC- -5' |
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| 22865 | 3' | -57.1 | NC_005137.2 | + | 87190 | 0.66 | 0.824559 |
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Target: 5'- cGACGGGCGcgGGCGGCuGCuCGUGcCGu- -3' miRNA: 3'- -UUGCUCGCa-CUGCCG-CG-GUACaGCuc -5' |
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| 22865 | 3' | -57.1 | NC_005137.2 | + | 75583 | 0.7 | 0.57881 |
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Target: 5'- uGGCGGacGCG-GGCGGCGCCGUGcgCGuGg -3' miRNA: 3'- -UUGCU--CGCaCUGCCGCGGUACa-GCuC- -5' |
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| 22865 | 3' | -57.1 | NC_005137.2 | + | 67724 | 0.67 | 0.751266 |
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Target: 5'- cGCGAGC--GACGGCGagaCCGUGUUGGa -3' miRNA: 3'- uUGCUCGcaCUGCCGC---GGUACAGCUc -5' |
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| 22865 | 3' | -57.1 | NC_005137.2 | + | 30761 | 0.67 | 0.798167 |
|
Target: 5'- -cCGuGCGUuGCGGCGCCcgGaUCGGu -3' miRNA: 3'- uuGCuCGCAcUGCCGCGGuaC-AGCUc -5' |
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| 22865 | 3' | -57.1 | NC_005137.2 | + | 27883 | 0.77 | 0.258127 |
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Target: 5'- uGGCGAucGCGUGGCGGCGCCAcggGUCa-- -3' miRNA: 3'- -UUGCU--CGCACUGCCGCGGUa--CAGcuc -5' |
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| 22865 | 3' | -57.1 | NC_005137.2 | + | 20630 | 0.66 | 0.824559 |
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Target: 5'- cAUGGGCGUGGUGGCGCUc-GUUGAa -3' miRNA: 3'- uUGCUCGCACUGCCGCGGuaCAGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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