miRNA: 3'- aUGAga--CA-UGCCGCCGUuaGuuUGUG- -5'
miRNA: 3'- augaGACAUGCCGC-----CGUUAGUuuGUG- -5'
miRNA: 3'- aUGAGACa---------UGCC-GCCG-UUAGUUugUG- -5'
miRNA: 3'- aUGAGA-----CA-UGCCGCCGu--UAGUUUGUG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22865 | 5' | -49.9 | NC_005137.2 | + | 812 | 0.67 | 0.986416 |
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Target: 5'- cGCUacagGUgGCGGCGGCAcgUuuACACc -3' miRNA: 3'- aUGAga--CA-UGCCGCCGUuaGuuUGUG- -5' |
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| 22865 | 5' | -49.9 | NC_005137.2 | + | 73120 | 0.66 | 0.992112 |
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Target: 5'- ---cCUGUACGGCGucgucGUAGUCGucCACg -3' miRNA: 3'- augaGACAUGCCGC-----CGUUAGUuuGUG- -5' |
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| 22865 | 5' | -49.9 | NC_005137.2 | + | 61359 | 0.66 | 0.992112 |
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Target: 5'- cGCUCUGcgucaaauggGCGGcCGGCgAGUUGAugGCg -3' miRNA: 3'- aUGAGACa---------UGCC-GCCG-UUAGUUugUG- -5' |
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| 22865 | 5' | -49.9 | NC_005137.2 | + | 13029 | 0.66 | 0.993186 |
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Target: 5'- aAUUCUccgccGUgACGGUGGUuucGUCGGACACg -3' miRNA: 3'- aUGAGA-----CA-UGCCGCCGu--UAGUUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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