Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22867 | 3' | -52.9 | NC_005137.2 | + | 103500 | 1.05 | 0.006841 |
Target: 5'- gGAAGCUAACCCACCCUGCAACGCAUUu -3' miRNA: 3'- -CUUCGAUUGGGUGGGACGUUGCGUAA- -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 46054 | 0.72 | 0.694394 |
Target: 5'- cGAGGUUAugCCGCCgCUGCAcaGCGUg-- -3' miRNA: 3'- -CUUCGAUugGGUGG-GACGU--UGCGuaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 108173 | 0.71 | 0.726078 |
Target: 5'- --cGCgcGCCCGCCCUcgcguuugGCAACGCGc- -3' miRNA: 3'- cuuCGauUGGGUGGGA--------CGUUGCGUaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 79726 | 0.68 | 0.850225 |
Target: 5'- --cGCU-GCCCGCCgcaaaUUGCGACGCAa- -3' miRNA: 3'- cuuCGAuUGGGUGG-----GACGUUGCGUaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 125742 | 0.66 | 0.932999 |
Target: 5'- --uGCuUGACCCGgCUUGCGccGCGCGUUu -3' miRNA: 3'- cuuCG-AUUGGGUgGGACGU--UGCGUAA- -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 32966 | 0.66 | 0.932999 |
Target: 5'- aAGGCgcugcugGACCCGCUC-GCAGCGCc-- -3' miRNA: 3'- cUUCGa------UUGGGUGGGaCGUUGCGuaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 50296 | 0.66 | 0.938196 |
Target: 5'- --cGC-GGCCCACCCUuGUAAUGUAa- -3' miRNA: 3'- cuuCGaUUGGGUGGGA-CGUUGCGUaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 59944 | 0.66 | 0.943128 |
Target: 5'- uGAGCUGGCgCGCCUcGUugauGCGCAUg -3' miRNA: 3'- cUUCGAUUGgGUGGGaCGu---UGCGUAa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 6015 | 0.66 | 0.947797 |
Target: 5'- -cGGCau-CCCGCCCgauuuccaacGCAGCGCAg- -3' miRNA: 3'- cuUCGauuGGGUGGGa---------CGUUGCGUaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 82506 | 0.66 | 0.947797 |
Target: 5'- cGGGC-GACCCGCUUUGCGAUaGCAa- -3' miRNA: 3'- cUUCGaUUGGGUGGGACGUUG-CGUaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 96414 | 0.66 | 0.947797 |
Target: 5'- --cGCU-ACCCGCgCgGCGACGCGc- -3' miRNA: 3'- cuuCGAuUGGGUGgGaCGUUGCGUaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 2006 | 0.66 | 0.947797 |
Target: 5'- -cAGCUcgUgCACgCUGCGGCGCAg- -3' miRNA: 3'- cuUCGAuuGgGUGgGACGUUGCGUaa -5' |
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22867 | 3' | -52.9 | NC_005137.2 | + | 2870 | 0.66 | 0.947797 |
Target: 5'- aGggGC-GACCCACuguaCCUGCAAC-CAc- -3' miRNA: 3'- -CuuCGaUUGGGUG----GGACGUUGcGUaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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