Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22869 | 3' | -50.5 | NC_005137.2 | + | 12508 | 0.66 | 0.992511 |
Target: 5'- aCGaUGGaCGGUCGgucuacggcgcACAUGAGCACgCCa- -3' miRNA: 3'- -GCaACC-GUUAGU-----------UGUACUCGUG-GGcu -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 99590 | 0.66 | 0.991375 |
Target: 5'- ---cGGC-GUCGACGUGcAGCugCCa- -3' miRNA: 3'- gcaaCCGuUAGUUGUAC-UCGugGGcu -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 111203 | 0.66 | 0.991375 |
Target: 5'- uGgcGGCAAUCuuuuuggGAGCACCgGGu -3' miRNA: 3'- gCaaCCGUUAGuugua--CUCGUGGgCU- -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 87039 | 0.66 | 0.9887 |
Target: 5'- ---aGGCGAguaCAACAUGGcgguggacGCGCCCGu -3' miRNA: 3'- gcaaCCGUUa--GUUGUACU--------CGUGGGCu -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 2436 | 0.66 | 0.98714 |
Target: 5'- gCGgUGGC---CGACGUGcccGCGCCCGAc -3' miRNA: 3'- -GCaACCGuuaGUUGUACu--CGUGGGCU- -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 95584 | 0.66 | 0.98714 |
Target: 5'- uGUUGGCGuuguaGUUAAUgAUGGGCGCgcgCCGAu -3' miRNA: 3'- gCAACCGU-----UAGUUG-UACUCGUG---GGCU- -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 63632 | 0.67 | 0.983529 |
Target: 5'- ----uGCAAUCAACAUG-GUGCCCa- -3' miRNA: 3'- gcaacCGUUAGUUGUACuCGUGGGcu -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 65272 | 0.68 | 0.971196 |
Target: 5'- cCGgcGGCGAcggUGACcauUGGGUACCCGAc -3' miRNA: 3'- -GCaaCCGUUa--GUUGu--ACUCGUGGGCU- -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 97989 | 0.7 | 0.929156 |
Target: 5'- ---gGGCGcgCGGCGUGGguGCGCCCGc -3' miRNA: 3'- gcaaCCGUuaGUUGUACU--CGUGGGCu -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 18007 | 0.7 | 0.928606 |
Target: 5'- aGUUGGCAuuuagccGUCGgggaugcgacaGCAUGAGCuguuACCCGu -3' miRNA: 3'- gCAACCGU-------UAGU-----------UGUACUCG----UGGGCu -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 24366 | 0.71 | 0.898424 |
Target: 5'- uGUUGGCAAUaaaCGugGUGAGCAaCUGGu -3' miRNA: 3'- gCAACCGUUA---GUugUACUCGUgGGCU- -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 104443 | 0.72 | 0.853199 |
Target: 5'- -cUUGGCAGUCGACAcGccgcAGCGCCaCGAc -3' miRNA: 3'- gcAACCGUUAGUUGUaC----UCGUGG-GCU- -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 27880 | 0.77 | 0.624239 |
Target: 5'- gGUUGGCGAUC-GCGUGgcGGCGCCaCGGg -3' miRNA: 3'- gCAACCGUUAGuUGUAC--UCGUGG-GCU- -5' |
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22869 | 3' | -50.5 | NC_005137.2 | + | 98755 | 1.09 | 0.007575 |
Target: 5'- uCGUUGGCAAUCAACAUGAGCACCCGAc -3' miRNA: 3'- -GCAACCGUUAGUUGUACUCGUGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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